Characterisation of bcl2-jh rearrangements in follicular lymphoma: pcr detection of 3′ bcl2 breakpoints and evidence of a new cluster

ABSTRACT Follicular lymphomas (FL) are closely associated with a t(14;18)(q32;q21) translocation, leading to a bcl2 protein overproduction. This translocation probably constitutes a very

early step in the development of the disease. Besides the cytogenetic assay, t(14;18) detection can be achieved using either Southern blot or polymerase chain reaction (PCR). Since 1990,

several publications have reported discrepancies between the results of cytogenetic and molecular analysis of t(14;18). Using methods able to explore long DNA fragments, several authors

reported breakpoints located outside the usual breakpoint regions. However, these techniques cannot be easily used in routine. The aim of this study was to develop a simple PCR assay to

amplify rearrangements usually not detected in FL. We selected a group of 83 patients with a t(14;18) on cytogenetic analysis: using usual probes and primers, 54/83 (65.1%) showed a MBR

rearrangement, 7/83 (8.4%) were mcr positive and 22/83 (26.5%) remained negative. Among these 22 rearrangements, nine could be detected using this new PCR assay. Four breakpoints were

located in a 20 bp area suggesting a recurrent breakpoint cluster close to an _Alu_ repetitive sequence. Finally, remaining negative cases (13/83, 15.6%) suggest that other breakpoints are

located between the MBR and mcr regions. _Leukemia_ (2000) 14, 1563–1569. Access through your institution Buy or subscribe This is a preview of subscription content, access via your

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Comité Départemental de Seine-Maritime de la Ligue Nationale Française Contre le Cancer. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * INSERM, EPI 9906, IFRMP No. 23, Génétique et

Hématologie Moléculaire, Centre Henri Becquerel, Rouen, France G Buchonnet, F Parmentier & C Bastard * Département d’Hématologie, Centre Henri Becquerel, Rouen, France P Lenain, S

Lepretre, A Stamatoullas, F Jardin & H Tilly * INSERM Unite 519, Centre Hospitalier et Universitaire, Rouen, France P Ruminy * Laboratoire d’Anatomie Pathologique, Centre Henri

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P., Ruminy, P. _et al._ Characterisation of _BCL2_-JH rearrangements in follicular lymphoma: PCR detection of 3′ _BCL2_ breakpoints and evidence of a new cluster. _Leukemia_ 14, 1563–1569

(2000). https://doi.org/10.1038/sj.leu.2401889 Download citation * Received: 18 April 2000 * Accepted: 25 May 2000 * Published: 31 August 2000 * Issue Date: 01 September 2000 * DOI:

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currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * follicular lymphoma * _BCL2_ * minimal residual disease

* PCR