Enrichment of bacillus subtilis transformants by zonal centrifugation

ABSTRACT A COMPETENT culture of _Bacillus subtilis_ which has been exposed to DNA consists of at least two metabolically distinct cell populations—competent cells which have taken up DNA and

are potentially transformable, and untransformable cells. The potential transformants are relatively resistant to penicillin, actinomycin _D_, and puromycin and appear to be non-dividing

(refs, 1–3 and personal communication from H. O. Kammen, R. H. Beloff, and E. S. Canellakis). We suspected that these cells were lighter than the majority of actively dividing incompetent

cells. Zonal centrifugation of a competent culture of _B. subtilis_ in a sucrose gradient shows that these two kinds of cells can, in fact, be separated. Access through your institution Buy

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MYTH AND REVEAL COLONY DEVELOPMENT Article Open access 07 July 2022 POOLED OPTICAL SCREENING IN BACTERIA USING CHROMOSOMALLY EXPRESSED BARCODES Article Open access 03 June 2025 REFERENCES *

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Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Molecular Biology Unit, Tata Institute of Fundamental Research, Bombay R. N. SINGH & M. P.

PITALE Authors * R. N. SINGH View author publications You can also search for this author inPubMed Google Scholar * M. P. PITALE View author publications You can also search for this author

inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE SINGH, R., PITALE, M. Enrichment of _Bacillus subtilis_ Transformants by Zonal

Centrifugation. _Nature_ 213, 1262–1263 (1967). https://doi.org/10.1038/2131262a0 Download citation * Issue Date: 25 March 1967 * DOI: https://doi.org/10.1038/2131262a0 SHARE THIS ARTICLE

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