Brca2 and rb1 loss responds to parp inhibitors

Access through your institution Buy or subscribe Molecular mechanisms and genomic consequences of _BRCA2_ and RB1 co-loss were prospectively investigated in prostate cancer cell lines.

CRISPR–Cas9 and RNA interference (RNAi) methods were used to eliminate these genes in LNCaP and LAPC4 cells. Loss of _BRCA2_ led to a castration-resistant phenotype; co-loss of both genes

induced epithelial-to-mesenchymal transition (EMT). However, PARP inhibitors attenuated cell growth in human metastatic castration-resistant prostate cancer (CRPC) organoids and CRPC cells

that had single-copy loss of both genes. Thus, identification of patients with co-loss of _RB1_ and _BRCA2_ could enable early use of PARP inhibition and improve outcomes. This is a preview

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* Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES ORIGINAL ARTICLE * Chakraborty, G. et al. Significance of _BRCA2_ and _RB1_ co-loss

in aggressive prostate cancer progression. _Clin. Cancer Res._ https://doi.org/10.1158/1078-0432.CCR-19-1570 (2020) Article  Google Scholar  RELATED ARTICLE * Taylor, R. A. et al. The

influence of _BRCA2_ mutation on localized prostate cancer. _Nat. Rev. Urol._ 16, 281–290 (2019) Article  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS *

Nature Reviews Urology http://www.nature.com/nrurol/ Annette Fenner Authors * Annette Fenner View author publications You can also search for this author inPubMed Google Scholar

CORRESPONDING AUTHOR Correspondence to Annette Fenner. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Fenner, A. _BRCA2_ and _RB1_ loss responds to PARP

inhibitors. _Nat Rev Urol_ 17, 132 (2020). https://doi.org/10.1038/s41585-020-0293-0 Download citation * Published: 11 February 2020 * Issue Date: March 2020 * DOI:

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