Ex vivo pulsed dendritic cell vaccination against cancer

ABSTRACT As the most powerful antigen-presenting cell type, dendritic cells (DCs) can induce potent antigen-specific immune responses in vivo, hence becoming optimal cell population for

vaccination purposes. DCs can be derived ex vivo in quantity and manipulated extensively to be endowed with adequate immune-stimulating capacity. After pulsing with cancer antigens in

various ways, the matured DCs are administrated back into the patient. DCs home to lymphoid organs to present antigens to and activate specific lymphocytes that react to a given cancer. Ex

vivo pulsed DC vaccines have been vigorously investigated for decades, registering encouraging results in relevant immunotherapeutic clinical trials, while facing some solid challenges. With

more details in DC biology understood, new theory proposed, and novel technology introduced (featuring recently emerged mRNA vaccine technology), it is becoming increasingly likely that ex

vivo pulsed DC vaccine will fulfill its potential in cancer immunotherapy. You have full access to this article via your institution. Download PDF SIMILAR CONTENT BEING VIEWED BY OTHERS A

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August 2020 INTRODUCTION Vaccination is a type of immunotherapy that is effective in the treatment of cancer. It provides the immune system with potential antigens for recognition and

usually activates antigen-specific lymphocytes via presentation of antigens by dendritic cells (DCs), which are the most adept cells regarding antigen uptake and processing. Activated

lymphocytes, especially T cells, assume effector functions such as cytotoxicity and cytokine production to controlling cancer progression. Before the prevalence of dendritic cell vaccines,

studies on cell-based vaccines focused on inactivated tumor cells that were engineered for enhanced immunogenicity. The so-called whole-cell cancer vaccines generally require antigen uptake

by endogenous DCs, which is a process that is relatively limited in efficiency. This strategy is reasonably advantageous because the antigens are delivered directly to DCs for maximum

efficacy, and this can be done either by ex vivo pulsing or in vivo targeting of DCs. Ex vivo pulsing of DCs is a process in which DCs that are derived from autologous origins are loaded

with antigens and matured under favorable ex vivo conditions. The resulting DCs are then administered back to the patient to initiate protective immune responses. Compared with in vivo

targeting, ex vivo pulsing of DCs has a lower risk, higher efficiency, and fewer technical difficulties. U.S. FDA approval of Sipuleucel-T, a vaccine against late-stage castration-refractory

prostate cancer and notably the first therapeutic DC vaccine against cancer, ushered cancer immunotherapy into a new era. Although numerous DC-based cancer vaccines have entered clinical

trials in recent years and have registered encouraging results, many issues have yet to be addressed. In this review, we discuss recent progress in and the future of vaccination with ex vivo

pulsed DCs against cancer, with an emphasis on emerging mRNA-pulsed DC vaccines. BIOLOGY OF DCS In 1973, Steinman and Cohn discovered a cell population with branching processes and named

these cell DCs after the Greek word for tree (dendreon) [1]. Decades of in-depth study of this heterogeneous population finally verified that they are the most potent professional

antigen-presenting cells, which bridge the gap between innate and adaptive immunity and play a key role in eliciting adaptive immune responses. Steinman was awarded the Nobel Prize in 2011

for the remarkable discovery of DCs. DCs exist in two sequential stages, immature and mature DCs (mDCs) after. Most DCs in the body are immature, and they populate peripheral nonlymphoid

tissues, specialize in antigen uptake, express high levels of phagocytosis-related receptors and low levels of costimulatory molecules, such as CD80 and CD86, as well as adhesion molecules,

such as ICAM, and exhibit weak antigen-presenting capacity; hence, these cells are relatively incompetent at activating T cells [2]. Immature DCs (iDCs) migrate to peripheral lymphoid organs

after ingestion of antigen and stimulation by inflammatory factors. Maturation proceeds during migration, resulting in DCs that express high levels of major histocompatibility complex (MHC)

molecules and costimulatory molecules, such as CD40, CD70, CD80, and CD86, and secrete interleukin 12 (IL-12), IL-6, TNF-α, and IP-10. Furthermore, mDCs express increased levels of C–C

chemokine receptor type 7, a chemokine receptor that is responsible for lymph node homing. These molecules are vital for effective T-cell activation [3]. mDCs present antigens to and

activate T cells in the lymph nodes. Full activation of naïve T cells depends on the orchestration of three distinct signals. DCs present peptide-MHC complexes to T cells for recognition by

specific T-cell receptors, and the CD3 complex transduces an antigen recognition signal (Signal 1) into T cells. Signal 2 is determined by a balance between costimulatory and coinhibitory

molecules on DCs (CD80, CD86, PD-L1/2, CD40, CD70, OX40L, 4-1BBL, etc.) and T cells (CD28, CTLA-4, PD-1, CD40L, CD27, OX40, 4-1BB, etc.). Signal 3 is from the cytokine environment, which

regulates the proliferation, differentiation, and immune memory of T cells (Fig. 1). For example, IL-12 secreted by DCs promotes Th1 (T helper 1) immune responses, while IL-23, IL-6, and

IL-1β stimulate Th17 immune responses [4,5,6]. Activated T cells, such as cancer-specific cytotoxic T lymphocytes (CTLs), leave peripheral lymphoid organs for cancer foci, where they exert

their anticancer function. Moreover, DCs interact with natural killer (NK) cells and further boost anticancer effects. Recently, it was found that mDCs enhance NK cell proliferation,

activation, and cytotoxicity through the interaction of DC-expressed CX3CL1 and NK cell-expressed CX3CR1, as well as through the secretion of cytokines, such as IL-12, IL-15, and IL-18 [7].

VACCINATION WITH EX VIVO PULSED DENDRITIC CELLS—THE BASICS AND THE REGULAR STORY PREPARATION OF DCS Despite their general presence in most tissues, the absolute numbers of DCs are low. For

example, mDCs account for only ~1% of total peripheral blood mononuclear cells (PBMCs). As ex vivo derivation of DCs improves, multiple precursor cells can be used to prepare DCs, such as

nonproliferative CD14+ monocytes from peripheral blood and proliferative CD34+ precursor cells from bone marrow and the umbilical blood [8, 9]. In addition, various cell types can be

redirected to a dendritic cell fate either by direct transdifferentiation or indirect dedifferentiation followed by redifferentiation (Fig. 2). CD14+ monocytes constitute ~10% of PBMCs, and

DCs derived from peripheral blood monocytes (MoDCs) have been extensively studied and applied. In 1994, Sallusto and Romani established a method for the induction of DCs from monocytes by

granulocyte–macrophage colony-stimulating factor (GM-CSF) and IL-4. GM-CSF sustains differentiation toward and subsequent development of DCs, while IL-4 suppresses the proliferation of

macrophages and granulocytes and prevents the differentiation of monocytes toward macrophages [10, 11]. Two years later, Romani and Zhou made an improved protocol public. They obtained iDCs

after induction for 6–7 days with GM-CSF and IL-4 and then developed mDCs after stimulation for 3 days with activating factors such as TNF-α. This method also registered the first successful

effort to replace bovine serum with human plasma in culture, which lays the foundation for the clinical application of ex vivo-derived DCs [12, 13]. To date, more ex vivo derivation

protocols of MoDCs have been explored, such as replacing IL-4 with IL-15 or interferon α (IFN-α) in the presence of GM-CSF to bolster the activation potency of DCs [14,15,16]. Apart from the

classical 1-week protocol, researchers invented fast protocols with which DCs could be harvested after 2–3 days of culture [17]. The maturation of DCs was commonly performed by adding the

gold standard cocktail (TNF-α, IL-1β, IL-6, and prostaglandin E2) to cultures [18]. Later, different teams developed somewhat similar modifications to the formulation (IFN-γ and LPS/MPLA)

[19, 20]. MoDCs cannot be propagated ex vivo; as a result, their application is somewhat limited. CD34+ hematopoietic stem/progenitor cells (HSPCs) can be used to prepare DCs in large

amounts ex vivo. These DCs (34DCs) are superior to MoDCs in that they elicit more potent T-cell immune responses against cancer by upregulating the expression of tumor necrosis

factor-related apoptosis-inducing ligand and enhancing cytotoxicity [21, 22]. Derivation of robust 34DCs was typically achieved in a cytokine milieu that differed from that for MoDC

derivation. The combination of fms-related tyrosine kinase 3 ligand (Flt3L), thrombopoietin (TPO), and stem cell factor (SCF) was one of the first documented formulations for such a purpose.

Alternatively, culturing in the presence of Flt3L, SCF, IL-3, and IL-6 for 3 weeks before switching to culture in the presence of Flt3L, TPO, and SCF for 1 week worked similarly well [23].

Recently, it was reported that the inclusion of Notch ligand Delta-like 1 (DLL1) in the original formulation of GM-CSF, Flt3L, and SCF significantly improved the induction of bona fide type

1 conventional DCs (cDC1s), which specialize in priming CD8+ CTLs, from CD34+ HSPCs [24]. Efforts to dissect the hematopoietic progenitor subsets that give rise to 34DCs, especially cDC1s,

revealed that, aside from the widely recognized common myeloid progenitor lineage, a large proportion of multipotent lymphoid progenitors share the same potential [25]. As the understanding

of the signals that govern cell differentiation continued to deepen, fresh strategies for deriving DCs were developed. Forced coexpression of PU.1, IRF8, and BATF3 transdifferentiated

fibroblasts into cDC1s that were competent for cell therapy [26]. Alternatively, various cell types can be efficiently dedifferentiated into induced pluripotent stem cells (iPSCs) before

being redifferentiated into DCs [27]. This process is a once-and-for-all solution that generates DCs in unprecedented quantities, although it is currently not time- or budget-friendly.

Recently, it was demonstrated that iPSCs derived from primary DCs were a superior source of DCs for immunotherapy, as the epigenetic imprints retained after dedifferentiation helped to

ensure high immunogenicity of the generated DCs [28]. PULSING OF DCS WITH CANCER ANTIGENS The pulsing of DCs with cancer antigens is the key step in preparing DC vaccines. DCs are usually

pulsed with whole-cell antigens from ultrasonicated or repeatedly frozen and thawed cancer samples, synthetic cancer antigenic peptides, DNA or RNA from cancer cells, and exosomes derived

from cancer cells [29,30,31,32] (Fig. 2). Tumor antigenic peptides are synthesized according to verified or predicted epitopes in cancer antigens and are used in many clinical trials to

pulse DCs. However, there are considerable weaknesses in this approach. For example, epitopes are restricted by the HLA (human leukocyte antigen) type of the patients, most cancer antigenic

epitopes are not yet elucidated, and peptides may only serve to elicit either CD4+ or CD8+ T-cell responses [33]. Furthermore, the half-life of the HLA-antigenic peptide complex is

relatively short, thus limiting the duration of presentation [34]. Exosomes from cancer cells carry abundant cancer antigens, and exosome-pulsed DCs are more efficacious than tumor lysates,

presumably because DNA in exosomes activates DCs through the cGAS/STING pathway and promotes DC maturation and presentation, thereby eliciting more potent immune responses against cancer

[35, 36]. However, it remains difficult to obtain large quantities of highly purified exosomes. Apart from the aforementioned method of loading, pulsing DCs ex vivo with mRNAs encoding

cancer antigens has emerged conspicuously in recent years, which will be discussed later in this review. CONVENTIONAL CLINICAL APPLICATIONS OF DC VACCINES DC vaccines have a similar clinical

objective response rate (ORR) to other conventional therapies against cancer. For example, the ORR of melanoma patients receiving DC immunotherapy is 8.5%, which is similar to the rate of

those receiving the first-line drug dacarbazine. Likewise, the ORRs of patients with prostate cancer, malignant glioma, and renal cell carcinoma receiving DC immunotherapy are 7.1%, 15.6%,

and 11.5%, respectively, which are similar to the rates of those receiving conventional chemotherapy [37]. In a phase III clinical trial of a DC vaccine for the treatment of glioblastoma,

all enrolled patients were randomly grouped after surgery and chemotherapy. The treatment group received the autologous DC vaccine (DCVax-L) and temozolomide, while the control group

received temozolomide and a placebo. DCVax-L was prepared from autologous DCs that were activated by lysates of surgically excised tumor tissues. The results indicated that the treatment

group achieved a median overall survival (mOS) of 23.1 months compared with a mOS of only 15–17 months for those who received surgery alone. In addition, 67 patients (30%) survived longer

than 30 months, 44 (24.2%) survived longer than 36 months, the regimen was well tolerated, with only 2.1% (7/331) of the patients exhibiting Grade III–IV adverse events [38]. Vaccination of

melanoma patients with DCs pulsed with mutated peptides of neoantigens enhanced T-cell immune responses directed against not only dominant neoantigens but also subdominant ones, thus

expanding the breadth of reaction and strengthening the potency of the vaccine [39]. Preconditioning with recall antigens such as Tetanus toxoid unilaterally before vaccination with DCs

pulsed with cancer antigens stimulated bilateral DC migration to lymph nodes that drained the vaccination sites, thereby enhancing the efficacy of DC vaccination. The effects depended on

both the CD4+ T-cell recall response to antigens used for preconditioning and the host CCL3 chemokine [40]. In a trial of 34DC immunotherapy of melanoma, 18 enlisted _HLA A*0201__+_ patients

were administered 34DCs pulsed with antigenic peptides of melanoma-associated tyrosinase, gp100, MART-1, and MAGE-3 antigens, along with those of control antigens. Sixteen patients

responded to at least one melanoma antigen, and ten responded to more than two melanoma antigens. Of the ten potential good responders, only one had progressive disease, and seven

experienced regression of metastases [41]. The results of these and many other studies [42,43,44] suggest that vaccination with ex vivo pulsed DCs is efficacious against cancer with minor,

if any, side effects. The anticancer efficacy of DC vaccines can be reinforced once procedures, such as loading DCs with tumor antigens, culture of DCs ex vivo and the route of

administration, are further optimized (Figs. 2, 3). NEW EMERGENCE OF MRNA-PULSED DC VACCINES AGAINST CANCER MRNA FOR DC PULSING mRNA is widely recognized as an ideal tool for the preparation

of DC vaccines [45, 46] because of its unique traits. mRNA does not integrate into the genome, avoiding any potential insertional mutagenesis. mRNA can be readily produced in large amounts

in vitro in a process that is both technologically mature and cost-efficient. mRNA can be engineered to increase immunogenicity and reduce inhibition of its translation. mRNA is degraded by

physiological mechanisms, facilitating the control of effects in a timed fashion. mRNA is not subject to splicing as pre-mRNA is, eliminating any uncertainty in protein products due to

alternative splicing. After the introduction of mRNA into DCs, specific T-cell responses targeting multiple epitopes can be elicited, mitigating the risk of immune evasion through antigen

variation [47]. Since mRNA-pulsed DC vaccines have stepped into the research spotlight, a paradigm shift away from DC vaccines pulsed in a conventional fashion is taking place. mRNA used for

DC pulsing includes cancer-derived and in vitro transcribed mRNA. Cancer-derived mRNA conveys the full repertoire of epitopes of a given cancer, expanding the range of antigens to which the

immune system responds, thus preventing evasion resulting from antigen downregulation or loss. However, preparing tumor-derived mRNA requires either a large number of tumor cells or

amplification of isolated mRNA. In either case, the majority of all mRNAs encode unaltered self-antigens, a small portion of which (tissue-specific, mutated, and aberrantly expressed ones)

are deemed as potentially appropriate targets for vaccination. The mRNAs that encode altered self-antigens and foreign antigens may become underrepresented or even lost during processing.

Moreover, the process is both time-consuming and laborious and is thus unfavorable for clinical application. mRNA encoding cancer antigens can be transcribed in vitro from templates of open

reading frame (ORF)-containing plasmids or other DNA fragments. Rational design of cap, 5′ and 3′ untranslated regions (UTRs) and poly (A) tail structure of mRNAs, and even the sorting

signals attached to the antigen, as well as the nucleotide sequence of the ORF itself and the introduction of modified nucleotides, strengthens mRNA stability, enhances translation, improves

antigen processing and presentation, avoids vigorous recognition by innate immune sensors, and culminates in augmented antigen-specific immune responses [48,49,50,51]. It has become

increasingly clear that apart from CD8+ T cells, CD4+ T cells, especially Th1 cells, are important participants in anticancer immunity [52]. mRNA-encoded proteins are synthesized in the

cytosol of DCs and are readily processed to antigenic peptides that then associate with MHC class I molecules and are presented to CD8+ T cells. However, activation of CD4+ T cells depends

on the MHC class II presentation pathway, which is not readily accessible to nonsecretory proteins in most cases. The usual solution to this problem is targeting these antigens to lysosomes

by means of fusion to lysosomal sorting signals so that both CD4+ and CD8+ arms of T-cell responses can be generated against cancer antigens [53, 54]. The strategies discussed above are far

from comprehensive, as the effects of DC vaccines are determined by multiple factors, such as the balance between costimulatory and coinhibitory molecules, as well as the balance between

activating and suppressive cytokines. In light of this, there was development of the TriMix formulation, which is a mixture of constitutively activated Toll-like receptor (TLR) 4, CD40L, and

CD70 mRNAs [55, 56]. The results of various preclinical and clinical studies on TriMix indicated that it boosted the immunostimulatory function of DCs. More importantly, education by

TriMix-DCs reprogrammed regulatory T cells (Tregs) to function like Th1 cells. An unprecedented ORR of 27% was recorded in Stage III/IV melanoma patients who were treated with TriMix-DCs

(NCT01066390) [57]. INTRODUCTION OF MRNA INTO DCS The pioneering effort of pulsing DCs with mRNAs encoding cancer antigens was developed by Boczkowski et al. at Duke University in the late

1990s [58]. In that study, mRNA was engulfed by DCs by macropinocytosis, which was problematic in that exogenous mRNA triggers signal transduction by the TLR-7 pattern recognition receptor

(PRR) pathway, and DCs activated by this signaling promptly curtail the ingestion of mRNA [59,60,61]. In addition, only a small fraction of mRNAs survived endosomal delivery, gained access

to the cytosol, and were translated into proteins. The methods of introducing mRNA into DCs have evolved significantly since then. Electroporation is probably the most widely adopted method

for the introduction of mRNA into DCs. Unlike DNA, mRNA does not have to enter the nucleus to exert its function. Therefore, a relatively weak electric pulse is sufficient for the delivery

of mRNA into the cytosol, greatly alleviating damage to the cells [62, 63]. In addition, since mRNA introduced by electroporation avoids the endosomal route where the mRNA-responsive PRRs

reside, unnecessary and potentially harmful recognition of mRNA by the innate immune system is avoided. Sonoporation exploits ultrasound to trigger the implosion of mRNA-loaded microbubbles

on target cells, thus forcing entry of mRNA into cells [64]. Nanofection delivers mRNA into cells in the form of a nanomaterial-mRNA complex, which typically travels via the endocytic

pathway and ultimately releases its cargo into the cytosol [65]. CLINICAL TRIALS OF MRNA-PULSED DC VACCINES AGAINST CANCER Numerous studies have confirmed that autologous DC vaccines

prepared with mRNAs encoding cancer antigens are both safe and efficacious. The last two decades witnessed more than 40 clinical trials adopting such a strategy (Table 1). In these trials,

mRNAs produced by in vitro transcription, as well as those derived from autologous cancers or cancer stem cells, were used to load DCs with cancer antigens. Aside from the origins of the

mRNAs, these trials also differed in activation tactics, varying from applying proinflammatory cytokines to co-delivering TriMix mRNAs. These DC vaccines were tested in the treatment of

ovarian cancer, mammary cancer, late-stage melanoma, leukemia, malignant glioma, mesothelioma, pancreatic cancer, esophageal cancer, myeloma, lung cancer, etc. For most trials, DC vaccines

were administered intradermally or intravenously, with some exceptions for intranodal and intratumoral administration. In a trial involving 15 late-stage melanoma patients (NCT01066390) who

received treatment of TriMix-DCs with mRNA encoding MAGE-A3, MAGE-C2, tyrosinase, and gp100 antigens (TriMix-DC-MEL), two achieved complete response, and another two achieved partial

response. In six of twelve patients, antigen-specific skin-infiltrating lymphocytes were detected, and in four of five patients, antigen-specific CD8+ T cells were detected in the blood

[57]. Also noteworthy is that recent trials tended to combine chemotherapy or antibody-targeted therapy with DC vaccines to strengthen efficacy (NCT00626483, NCT02649829, NCT02366728, and

NCT02649582). In a phase I trial of autologous Langerhans-type DCs pulsed with xenogeneic TRP-2 mRNA, stage IIB to IV melanoma patients who had their tumors resected were vaccinated 5 times

at 2-week intervals. Six out of nine participants stayed disease-free for a median of 51.1 months. The patients developed clinical outcome-related immune responses, including activation and

increased clonality of T cells and secretion of proinflammatory cytokines, and showed minimal signs of toxicity [66]. In addition, enhanced antitumor activities were also observed in

combinatorial treatment with mRNA-pulsed DCs and immune checkpoint inhibitors. In 2011, a phase II trial for the treatment of unresectable stage III/IV melanoma with TriMix-DCs and the

anti-CTLA-4 monoclonal antibody ipilimumab (NCT01302496) was performed, in which the 6-month disease control rate was 51%, the overall response rate was 38% (eight complete responses and

seven partial responses), and there were still seven complete responses and one partial tumor response after a median follow-up time of 36 months [67]. OUTLOOK DCs play pivotal roles in

initiating adaptive immune responses, and exploiting DCs for anticancer therapy is a promising strategy. However, it still faces challenges that call for imperative improvements. Functional

impairments were observed in endogenous DCs in peripheral blood and the tumor-draining lymph nodes of cancer patients [68], as well as DCs derived ex vivo from monocytes of cancer patients

[69]. These dysfunctions were only partially reverted by tumor resection [68] or transforming growth factor β (TGF-β) blockade [69], suggesting that further countermeasures must be taken to

erase adverse imprints left by tumors on DCs and DC precursors. Culture of DCs ex vivo offers a unique window of time in which extensive direct interventions can be implemented in DCs to

correct their functional defects. It was estimated that as few as 85 DCs are sufficient for stimulating a T-cell immune response [70]. The recruitment of naive T cells is highly efficient

[71]. It might be slightly counterintuitive that DC vaccination involving the administration of a large amount of pulsed DCs has resulted in relatively limited success. DC subsets,

derivation protocols, activation status, antigen loading, route of administration, vaccination schedules, mitigation of immunosuppression by cancer, etc. are all factors that determine the

outcome of DC vaccination (Fig. 3). DCs are heterogeneous cell populations that share similar characteristics while varying in origins and detailed functions. For most preclinical studies of

DC vaccination, the subjects are bone marrow-derived DCs (BMDCs), and for most clinical trials, MoDCs are frequently used. These subsets, while from different species, are both believed to

be counterparts of inflammatory DCs that exist in vivo rather than resident DCs [72, 73]. Inflammatory DCs act as replacements or complements of migratory cDC1s during inflammation [74] and

can present antigens by themselves or transfer antigens to lymphoid tissue-resident DCs for efficient presentation. The latter process is important, as not all migratory DCs are well

equipped for T-cell priming by themselves [75, 76]. Therefore, it might be worth attempting to derive DCs that resemble lymphoid-tissue resident DCs for vaccination purposes [77] and further

discern which DC subset is most appealing to use in vaccinations. In fact, the notion of the MoDC vaccine was challenged from inside by a recent study asserting that vaccines based on

undifferentiated monocytes were superior to DC vaccines in terms of anticancer efficacy in multiple murine tumor models. The anticancer efficacy depended on peptide transfer from monocytes

to CD8+ splenic DCs (murine equivalents of human CD141+ DCs in terms of cross-presentation activity [78]) through connexin 43 gap junctions for presentation. However, connexin 43 gap

junctions were permeable to peptides of no more than 11 amino acid residues; therefore, trafficking of only MHC class I-restricted rather than MHC class II-restricted peptides through such

junctions was possible [79]. The extent of and the mechanisms by which CD4+ T-cell responses are elicited by monocyte-based cellular vaccines have to be fully elucidated before this new

vaccine formulation can impact cancer immunotherapy. Current designs place elements from α and/or β globin genes in the 5′ and/or 3′ UTR of mRNAs to eventually increase protein productivity

[60, 80, 81]; however, by exploring the repertoire of cellular and viral elements and with the help of computer-aided design, more powerful factors might be discovered for this purpose. In

addition, many genes exert their influence on cancer immunity, and so it is expected that modulation of their expression and function by introduction of mRNAs other than those components of

TriMix might trigger even more effective anticancer immune responses. One cannot expect cancers to be easily controlled or even eradicated solely by a single approach. Combinatorial regimens

have been and will always be a topic of heated investigation. As illustrated in Fig. 3, induction of stronger immune responses against cancer antigens and amelioration of the tumor

microenvironment to facilitate infiltration and functioning of immune cells (activated T included) act in synergy to eventually attain pronounced regression of tumor nodules and elimination

of micrometastases. Recent studies of combinatorial therapy marked the latest efforts toward that ultimate goal [82,83,84,85,86]. The CRISPR base editing system guides deaminases to specific

genomic locations and changes DNA sequences, thus abrogating the expression of specific genes without causing double-stranded breaks [87]. With this powerful genetic tool, DCs can be

liberated from suppression by various negative regulators, such as the SOCS family, TGF-β receptor, and IL-10 receptor. This can be done ex vivo by introduction of relevant ribonucleoprotein

or the combination of relevant mRNA and sgRNA (single guide RNA) into DCs. Hopefully, increasingly potent DC vaccines can be generated through this modification process. Ex vivo pulsed DC

vaccines are an important platform into which new ideas and technologies have been continuously introduced. Hopes are high that, by bringing in these impetuses, DC vaccines will be properly

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applications. Trends Biotechnol. 2019;37:1121–42. CAS  PubMed  Google Scholar  Download references ACKNOWLEDGEMENTS This work was supported by the National Natural Science Foundation of

China (81872821) and the National Key S&T Special Projects (2018ZX09201018-024). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * State Key Laboratory of Biotherapy and Cancer Center, West

China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China Yang-zhuo Gu, Xing Zhao & Xiang-rong Song * Stem Cell and Tissue

Engineering Research Center, Guizhou Medical University/Key Laboratory of Adult Stem Cell Transformation Research, Department of Immunology, Chinese Academy of Medical Sciences, Guiyang,

550004, China Xing Zhao Authors * Yang-zhuo Gu View author publications You can also search for this author inPubMed Google Scholar * Xing Zhao View author publications You can also search

for this author inPubMed Google Scholar * Xiang-rong Song View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to

Xiang-rong Song. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing interests. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Gu,

Yz., Zhao, X. & Song, Xr. Ex vivo pulsed dendritic cell vaccination against cancer. _Acta Pharmacol Sin_ 41, 959–969 (2020). https://doi.org/10.1038/s41401-020-0415-5 Download citation *

Received: 15 December 2019 * Accepted: 30 March 2020 * Published: 04 May 2020 * Issue Date: July 2020 * DOI: https://doi.org/10.1038/s41401-020-0415-5 SHARE THIS ARTICLE Anyone you share

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Nature SharedIt content-sharing initiative KEYWORDS * cancer immunotherapy * dendritic cells * cancer antigens * DC vaccination * mRNA-pulsed DC vaccines * T-cell activation * tumor

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