Effects of tissue factor induced by oxygen free radicals on coronary flow during reperfusion

ABSTRACT Tissue factor is a transmembrane protein that activates the extrinsic coagulation pathway by binding factor VII. Endothelial cells, being in contact with circulating blood, do not

normally express tissue factor. Here we provide evidence that oxygen free radicals induce tissue factor messenger RNA transcription and expression of tissue factor procoagulant activity in

endothelial cells in culture. Isolated, perfused rabbit hearts exposed to exogenous oxygen free radicals also showed a marked increase in tissue factor activity within the coronary

circulation. Furthermore, in _ex vivo_ and _in vivo_ hearts subjected to ischemia and reperfusion, a condition associated with a production of oxygen free radicals in large amounts, a marked

increase in tissue factor activity occurred. This phenomenon could be abolished by oxygen radical scavengers. This increase in tissue factor activity during postischemic reperfusion was

accompanied by a significant decrease in coronary flow, suggesting that increase in tissue factor activity with the consequent activation of the coagulation cascade might impair coronary

flow during reperfusion and possibly contribute to the occurrence of reperfusion injury. Access through your institution Buy or subscribe This is a preview of subscription content, access

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references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Internal Medicine, Division of Cardiology, Centro di Endocrinologia ed Oncologia Sperimentale del Consiglio, Nazionale

dalle Ricerche, Second School of Medicine, University of Naples, Naples, Italy Paolo Golino, Massimo Ragni, Plinio Cirillo, Nicolino Esposito, Annalisa Scognamiglio, Bruno Trimarco, Guido

Iaccarino, Mario Condorelli & Massimo Chiariello * Department of Biology and Molecular and Cellular Pathology, Centro di Endocrinologia ed Oncologia Sperimentale del Consiglio, Nazionale

dalle Ricerche, Second School of Medicine, University of Naples, Naples, Italy Antonio Feliciello * Department of Experimental Medicine, School of Medicine at Catanzaro, University ofReggio

Calabria, Italy V. Enrico Avvdimento * Division of Cardiology, University of Perugia School of Medicine, Perugia, Italy Giuseppe Ambrosio Authors * Paolo Golino View author publications You

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P. _et al._ Effects of tissue factor induced by oxygen free radicals on coronary flow during reperfusion. _Nat Med_ 2, 35–40 (1996). https://doi.org/10.1038/nm0196-35 Download citation *

Received: 04 April 1995 * Accepted: 09 November 1995 * Issue Date: 01 January 1996 * DOI: https://doi.org/10.1038/nm0196-35 SHARE THIS ARTICLE Anyone you share the following link with will

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