Gene expression analysis of glucocorticoid-induced apoptosis by a gene microarray technique

You have full access to this article via your institution. Download PDF Glucocorticosteroids induce apoptosis in immature lymphocytes and are therefore commonly used to treat patients who

have lymphoid leukemia or lymphoma. To understand the genetic pathways and molecular mechanisms of glucocorticoid-induced apoptosis, we used the gene microarray technique to analyze gene

expression in a well-established mouse lymphoma cell line, S49.1. In initial experiments S49.1 cells were treated with the glucocorticoid hormone, dexamethasone or vehicle for 12 h, and

total RNA was extracted. Biotinylated antisense RNAs were hybridized to Affymetrix mouse U74 sub A chips, which contain about 12,000 known genes and expressed sequence tags. Following

dexamethasone treatment, 69 genes had variances greater than 2-fold, and 40 genes had variances greater than 2.5-fold. Among these 40 genes, 30 were induced and 10 were repressed in the

dexamethasone-treated cells. Included among the known genes with altered expression were members of the immunoglobulin superfamily, transcription factors and receptors coupled to G protein.

Among the expressed sequence tags with altered expression were tags with a high degree of homology to potassium channel proteins and β-catenins or other new genes. Earlier time points of the

gene expression patterns will be analyzed by the same approach in anticipation of finding other genes with potential involvement in the apoptosis pathway induced by glucocorticoids. AUTHOR

INFORMATION AUTHORS AND AFFILIATIONS * Case Western Reserve University, Cleveland, Ohio, USA Huiling He, Michael Malone, Steven Ostrowski & Clark Distelhorst Authors * Huiling He View

author publications You can also search for this author inPubMed Google Scholar * Michael Malone View author publications You can also search for this author inPubMed Google Scholar * Steven

Ostrowski View author publications You can also search for this author inPubMed Google Scholar * Clark Distelhorst View author publications You can also search for this author inPubMed 

Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE He, H., Malone, M., Ostrowski, S. _et al._ Gene expression analysis of

glucocorticoid-induced apoptosis by a gene microarray technique. _Nat Genet_ 27 (Suppl 4), 58 (2001). https://doi.org/10.1038/87108 Download citation * Issue Date: April 2001 * DOI:

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