Correcting crispr for copy number

The CRISPR–Cas9 system enables global screens of gene function with high sensitivity and specificity, but off-target effects have been reported for CRISPR guide RNAs targeting genes that are

amplified at high copy number. A new study describes a computational approach to correct for this copy number effect, increasing the specificity of CRIPSR screens to identify essential

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33, 661–667 (2015). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * the Department of Medicine, University of California, San Diego, La Jolla,

California, USA John Paul Shen & Trey Ideker Authors * John Paul Shen View author publications You can also search for this author inPubMed Google Scholar * Trey Ideker View author

publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Trey Ideker. ETHICS DECLARATIONS COMPETING INTERESTS T.I. is a cofounder of

Data4Cure, Inc., and has equity interest. T.I. has an equity interest in Ideaya Biosciences, Inc. The terms of these arrangements have been reviewed and approved by the University of

California, San Diego, in accordance with its conflict-of-interest policies. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Shen, J., Ideker, T.

Correcting CRISPR for copy number. _Nat Genet_ 49, 1674–1675 (2017). https://doi.org/10.1038/ng.3994 Download citation * Published: 29 November 2017 * Issue Date: 01 December 2017 * DOI:

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