System-wide molecular evidence for phenotypic buffering in arabidopsis

ABSTRACT We profiled 162 lines of _Arabidopsis_ for variation in transcript, protein and metabolite abundance using mRNA microarrays, two-dimensional polyacrylamide gel electrophoresis, gas

chromatography time-of-flight mass spectrometry, liquid chromatography quadrupole time-of-flight mass spectrometry, and proton nuclear magnetic resonance. We added all publicly available

phenotypic data from the same lines and mapped quantitative trait loci (QTL) for 40,580 molecular and 139 phenotypic traits. We found six QTL hot spots with major, system-wide effects,

suggesting there are six breakpoints in a system otherwise buffered against many of the 500,000 SNPs. Access through your institution Buy or subscribe This is a preview of subscription

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ACKNOWLEDGEMENTS This research was supported by the Netherlands Organization for Scientific Research (NWO), the Netherlands Genomics Initiative (NGI) and the UK Biotechnology and Biological

Sciences Research Council (BBSRC). AUTHOR INFORMATION Author notes * Jingyuan Fu and Joost J B Keurentjes: These authors contributed equally to this work. AUTHORS AND AFFILIATIONS *

Groningen Bioinformatics Centre, University of Groningen, 9751NN Haren, The Netherlands., Jingyuan Fu, Martijn Dijkstra, Richard A Scheltema, Frank Johannes, Rainer Breitling & Ritsert C

Jansen * Department of Genetics, University Medical Centre Groningen, University of Groningen, 9700RB Groningen, The Netherlands., Jingyuan Fu & Ritsert C Jansen * Laboratory of

Genetics, Wageningen University, 6703BD Wageningen, The Netherlands., Joost J B Keurentjes & Maarten Koornneef * Laboratory of Plant Physiology, Wageningen University, 6703BD Wageningen,

The Netherlands., Joost J B Keurentjes, Harro Bouwmeester, Francel W A Verstappen & Dick Vreugdenhil * Centre for Biosystems Genomics, Wageningen, 6708PB Wageningen, The Netherlands.,

Joost J B Keurentjes, Harro Bouwmeester, Twan America, Francel W A Verstappen & Ric C H de Vos * Plant Research International, Wageningen, 6708PB Wageningen, The Netherlands., Harro

Bouwmeester, Twan America, Francel W A Verstappen & Ric C H de Vos * Rothamsted Research, National Centre for Plant and Microbial Metabolomics, Harpenden, AL5 2JQ, Herts, UK Jane L Ward 

& Michael H Beale * Max Planck Institute for Plant Breeding Research, Cologne, 50829, Germany Maarten Koornneef Authors * Jingyuan Fu View author publications You can also search for

this author inPubMed Google Scholar * Joost J B Keurentjes View author publications You can also search for this author inPubMed Google Scholar * Harro Bouwmeester View author publications

You can also search for this author inPubMed Google Scholar * Twan America View author publications You can also search for this author inPubMed Google Scholar * Francel W A Verstappen View

author publications You can also search for this author inPubMed Google Scholar * Jane L Ward View author publications You can also search for this author inPubMed Google Scholar * Michael H

Beale View author publications You can also search for this author inPubMed Google Scholar * Ric C H de Vos View author publications You can also search for this author inPubMed Google

Scholar * Martijn Dijkstra View author publications You can also search for this author inPubMed Google Scholar * Richard A Scheltema View author publications You can also search for this

author inPubMed Google Scholar * Frank Johannes View author publications You can also search for this author inPubMed Google Scholar * Maarten Koornneef View author publications You can also

search for this author inPubMed Google Scholar * Dick Vreugdenhil View author publications You can also search for this author inPubMed Google Scholar * Rainer Breitling View author

publications You can also search for this author inPubMed Google Scholar * Ritsert C Jansen View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS

J.F. analyzed the system-wide data, J.J.B.K. managed the system-wide data production, H.B. and F.W.A.V. produced the GC-MS data, T.A. produced the 2D-PAGE data, J.L.W. and M.H.B. produced

the NMR data, R.C.H.d.V. produced the LC-MS data, M.D. and R.A.S. helped analyze the GC- and LC-MS data, F.J. helped analyze the NMR data, M.K. coordinated the QTL-Express project and

provided the biological materials, D.V. contributed to the biological interpretation, R.B. contributed to the system-wide interpretation and writing of the manuscript, R.C.J. conceived the

project and coordinated the analysis and writing of the manuscript. CORRESPONDING AUTHOR Correspondence to Ritsert C Jansen. SUPPLEMENTARY INFORMATION SUPPLEMENTARY TEXT AND FIGURES

Supplementary Table 1, Supplementary Figures 1–5 and Supplementary Methods (PDF 1433 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Fu, J.,

Keurentjes, J., Bouwmeester, H. _et al._ System-wide molecular evidence for phenotypic buffering in _Arabidopsis_. _Nat Genet_ 41, 166–167 (2009). https://doi.org/10.1038/ng.308 Download

citation * Received: 17 June 2008 * Accepted: 10 November 2008 * Published: 25 January 2009 * Issue Date: February 2009 * DOI: https://doi.org/10.1038/ng.308 SHARE THIS ARTICLE Anyone you

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