Interrogating Lkb1 function | Nature Cell Biology

Interrogating Lkb1 function | Nature Cell Biology


Play all audios:

Loading...

Access through your institution Buy or subscribe The kinase Lkb1 regulates cell polarity and is mutated or deleted in many cancers. Lkb1 substrates include the kinase AMPK, but whether AMPK


is a relevant Lkb1 target in polarity and tumorigenesis remains unclear. Mellman and colleagues now use elegant chemical genetics approaches to address this issue (_J. Cell Biol._ 199,


1117–1130; 2012). The authors generated a knock-in mouse expressing an analogue-sensitive kinase allele (ASKA) of Lkb1; this strategy enables Lkb1 to be inhibited by the compound 1NMPP1.


Mice homozygous for mutant Lkb1 were embryonic lethal, but embryonic tissues harvested from these mice could be used for _ex vivo_ analyses. Acute Lkb1 inhibition in the lung led to


branching defects but not loss of apical–basal polarity. Pancreatic explants similarly retained apical–basal polarity following Lkb1 inhibition, but formed dynamic cysts lined with rapidly


proliferating epithelial cells. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12


print issues and online access $209.00 per year only $17.42 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be


subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support Authors *


Emily J. Chenette View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE


Chenette, E. Interrogating Lkb1 function. _Nat Cell Biol_ 15, 141 (2013). https://doi.org/10.1038/ncb2692 Download citation * Published: 01 February 2013 * Issue Date: February 2013 * DOI:


https://doi.org/10.1038/ncb2692 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently


available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative