Import of adenovirus dna involves the nuclear pore complex receptor can/nup214 and histone h1

ABSTRACT Adenovirus type 2 (Ad2) imports its DNA genome through the nuclear pore complex (NPC) of cells in interphase for viral production. Here we identify the NPC-filament protein

CAN/Nup214 as a docking site for incoming Ad2 capsids. Binding to CAN is independent of cytosolic factors. Capsids disassemble at NPCs to free their DNA for import. This process requires

binding of nuclear histone H1 to the stably docked capsids and involves H1-import factors, restricting this irreversible process to the proximity of the nucleus. Our results provide a

molecular mechanism for disassembly of Ad2 and reveal an unexpected function of histone H1 in virus-mediated DNA import. Access through your institution Buy or subscribe This is a preview of

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  Google Scholar  Download references ACKNOWLEDGEMENTS We thank P. Groscurth for access to the confocal laser scanning microscope and P. Sonderegger for access to the SMART system, U.

Ziegler and P. Cinelli for assistance and U. Aebi, U. Kutay and T. Misteli for comments on the manuscript. Numerous investigators are gratefully acknowledged for their gifts of reagents (and

are indicated in the Methods section) and H. van der Velde is acknowledged for preparation of C terminus CAN/Nup214. The work was supported by the Swiss National Science Foundation and the

Kanton of Zürich. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * University of Zürich, Institute of Zoology, Winterthurerstrasse 190, Zürich, CH-8057, Switzerland Lloyd C. Trotman, Nicole

Mosberger, Robert P. Stidwill & Urs F. Greber * Netherlands Cancer Institute, Plesmanlaan 121, CX, 1066, Amsterdam, The Netherlands Maarten Fornerod Authors * Lloyd C. Trotman View

author publications You can also search for this author inPubMed Google Scholar * Nicole Mosberger View author publications You can also search for this author inPubMed Google Scholar *

Maarten Fornerod View author publications You can also search for this author inPubMed Google Scholar * Robert P. Stidwill View author publications You can also search for this author

inPubMed Google Scholar * Urs F. Greber View author publications You can also search for this author inPubMed Google Scholar SUPPLEMENTARY INFORMATION SUPPLEMENTARY FIGURES Figure S1

Microinjected anti-NPC antibodies reduce nuclear import of FITC–BSA–NLS. (PDF 614 kb) Figure S2 Histone H1 import factors are required for nuclear import of Ad2–DNA. RIGHTS AND PERMISSIONS

Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Trotman, L., Mosberger, N., Fornerod, M. _et al._ Import of adenovirus DNA involves the nuclear pore complex receptor CAN/Nup214

and histone H1. _Nat Cell Biol_ 3, 1092–1100 (2001). https://doi.org/10.1038/ncb1201-1092 Download citation * Received: 01 May 2001 * Revised: 07 August 2001 * Accepted: 19 September 2001 *

Published: 02 November 2001 * Issue Date: 01 December 2001 * DOI: https://doi.org/10.1038/ncb1201-1092 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

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