Reply: complex kinase requirements for epec pedestal formation

Access through your institution Buy or subscribe Hayward _et al_. reply: Our recent Letter1 and the report of Swimm _et al_.2 provide intriguing new insights into actin pedestal formation by

EPEC. Both groups investigated how the receptor (Tir) of this important human pathogen is tyrosine phosphorylated: we defined a specific requirement for the Src-family kinase (SFK) c-Fyn

using “priming and challenge” and _in vitro_ assays to uncouple Tir delivery and receptor clustering, whereas Swimm _et al_. investigated the potential pharmaceutical application of

pyrido[2,3-d]pyrimidine inhibitors using only a single EPEC wild-type strain (EPEC-WT). Their data implicated several redundant kinases, including SFKs, c-Abl and c-Arg; Swimm _et al_.

comment that their particular EPEC-WT forms pedestals on _src__−/−__ yes__−/−__ fyn__−/−_ (SYF) cells, which seems to be in contrast to our findings. This is a preview of subscription

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ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Phillips, N., Hayward, R.D. & Koronakis, V. _Nature Cell Biol._

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Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK Richard D. Hayward, Neil Phillips 

& Vassilis Koronakis Authors * Richard D. Hayward View author publications You can also search for this author inPubMed Google Scholar * Neil Phillips View author publications You can

also search for this author inPubMed Google Scholar * Vassilis Koronakis View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints

and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Hayward, R., Phillips, N. & Koronakis, V. Reply: Complex kinase requirements for EPEC pedestal formation. _Nat Cell Biol_ 6, 795–796

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