The targeted inhibitory effects of human amniotic fluid stem cells carrying cxcr4 promoter and dal-1 on non-small cell lung carcinoma growth

ABSTRACT The differentially expressed in adenocarcinoma of the lung-1 (DAL-1) protein has been demonstrated to be suppressive to various types of tumors including lung cancer. This study

aimed to determine the targeted effects of human amniotic fluid stem cells (hAFS cells) carrying CXCR4 promoter driven conditionally replicable adenovirus vector overexpressing DAL-1

(Ad-CXCR4-DAL-1) on non-small cell lung carcinoma (NSCLC) growth. The apoptotic effects of virus vectors were assessed using flow cytometry, and the cytotoxicity analyzed by CCK-8 assay. _In

vivo_ imaging system was used to determine the homing capability of hAFS cells. A549 cell xenograft mouse model was created to assess the _in vivo_ effect of DAL-1 overexpression on NSCLC

growth. We found that infection of Ad-CXCR4-DAL-1 increased the apoptosis of A549 NSCLC cells but not 16HBE normal human bronchial epithelial cells. Ad-CXCR4-DAL-1 administered via

intratumoral injection led to significant reduced growth and greater necrosis of A549 xenograft tumors comparing to null vector treated animals. When infused via tail vein, hAFS cells

carrying Ad-CXCR4-DAL-1 homed to lung cancer xenografts, caused virus replication and DAL-1 overexpression, and led to significant lower growth and greater necrosis of A549 cell xenografts

comparing to non-treatment control. In conclusion, hAFS cells are capable of carrying Ad-CXCR4-DAL-1 vectors, specifically targeting to lung cancer, and causing oncolytic effects when

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Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS ANTI-CANCER EFFECTS OF HUMAN PLACENTA-DERIVED AMNIOTIC EPITHELIAL STEM CELLS LOADED WITH PACLITAXEL ON CANCER CELLS Article

Open access 28 October 2022 IN VIVO INDUCTION OF ACTIVIN A-PRODUCING ALVEOLAR MACROPHAGES SUPPORTS THE PROGRESSION OF LUNG CELL CARCINOMA Article Open access 17 January 2023 EFFECTS OF

DEXMEDETOMIDINE ON A549 NON-SMALL CELL LUNG CANCER GROWTH IN A CLINICALLY RELEVANT SURGICAL XENOGRAFT MODEL Article Open access 01 August 2023 REFERENCES * Dresler C . The changing epidemic

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ACKNOWLEDGEMENTS This study was funded by the Education Department of China for Doctorate Advisor (20134423110001), Guangdong Natural Science Foundation (S2012010010181), Guangzhou Science

and Technology Scheme (2014Y2-00171), and Guangzhou Department of Education for Innovative Academic Team (13C06) grants. AUTHOR INFORMATION Author notes * L Li and S Li: These authors

contributed equally to this work. AUTHORS AND AFFILIATIONS * Center for Respiratory Pathology, State Key Lab of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical

University, Guangzhou, People’s Republic of China L Li * Department of Pathology, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, People’s Republic of China S Li, 

T Cai, H Wang, X Xie, Z Liu & Y Zhang Authors * L Li View author publications You can also search for this author inPubMed Google Scholar * S Li View author publications You can also

search for this author inPubMed Google Scholar * T Cai View author publications You can also search for this author inPubMed Google Scholar * H Wang View author publications You can also

search for this author inPubMed Google Scholar * X Xie View author publications You can also search for this author inPubMed Google Scholar * Z Liu View author publications You can also

search for this author inPubMed Google Scholar * Y Zhang View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Y Zhang.

ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no conflict of interest. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Li, L., Li, S., Cai,

T. _et al._ The targeted inhibitory effects of human amniotic fluid stem cells carrying CXCR4 promoter and DAL-1 on non-small cell lung carcinoma growth. _Gene Ther_ 23, 214–222 (2016).

https://doi.org/10.1038/gt.2015.90 Download citation * Received: 30 December 2014 * Revised: 11 May 2015 * Accepted: 05 August 2015 * Published: 17 August 2015 * Issue Date: February 2016 *

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