Ability of alpha-lipoic acid to reverse the diabetic cystopathy in a rat model

ABSTRACT AIM: The present study was conducted to investigate whether alpha-lipoic acid (α-LA) is able to reverse impaired bladder function induced by diabetes in a rat model and to explore

the possible mechanism mediating the effect. METHODS: Male Sprague-Dawley rats were divided randomly into 3 age-matched groups: control, diabetes mellitus (DM) treated with vehicle, and DM

with α-LA treatment. The diabetic rats were induced by a single intraperitoneal (ip) injection of streptozotocin (60 mg/kg). Six weeks after the induction of DM, the two groups received

another 6 weeks of treatment with vehicle or α-LA (100 mg/kg, ip). Body weight and blood glucose levels were measured weekly. The bladder function was evaluated by _in vitro_ cystometry. The

oxidative stress status was determined by biochemical methods, and the level of nerve growth factor was investigated by enzyme-linked immunosorbent assay. RESULTS: The

streptozotocin-induced diabetic rats showed impaired bladder function characterized by increased bladder capacity, decreased bladder contractility (voiding efficiency), and an increase in

residual urine. Treatment with α-LA significantly normalized the increased bladder capacity for inducing voiding, single-voided volume, and post-void residual volume. α-LA treatment

significantly reversed the increased level of malondialdehyde and reduced the activities of both superoxide dismutase and catalase. DM caused a decrease in the bladder nerve growth factor

(NGF) level, and α-LA upregulated the level of NGF in the diabetic rat bladder. CONCLUSION: These results indicate that α-LA has a beneficial effect on diabetes-induced cystopathy by

ameliorating oxidative stress and normalizing the NGF level in the bladder. SIMILAR CONTENT BEING VIEWED BY OTHERS TETRAHYDROBIOPTERIN PREVENTS CHRONIC ISCHEMIA-RELATED LOWER URINARY TRACT

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AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Urology, the First Affiliated Hospital, China Medical University, Shenyang, 110001, China Yuan-jun Jiang, Da-xin Gong, Hai-bo Liu,

 Chun-ming Yang, Zhi-xi Sun & Chui-ze Kong Authors * Yuan-jun Jiang View author publications You can also search for this author inPubMed Google Scholar * Da-xin Gong View author

publications You can also search for this author inPubMed Google Scholar * Hai-bo Liu View author publications You can also search for this author inPubMed Google Scholar * Chun-ming Yang

View author publications You can also search for this author inPubMed Google Scholar * Zhi-xi Sun View author publications You can also search for this author inPubMed Google Scholar *

Chui-ze Kong View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Chui-ze Kong. ADDITIONAL INFORMATION Project

supported by a grant from the Education Department of Liaoning Province (No 05L552). RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Jiang, Yj., Gong,

Dx., Liu, Hb. _et al._ Ability of alpha-lipoic acid to reverse the diabetic cystopathy in a rat model. _Acta Pharmacol Sin_ 29, 713–719 (2008).

https://doi.org/10.1111/j.1745-7254.2008.00790.x Download citation * Received: 14 December 2007 * Accepted: 25 February 2008 * Issue Date: 01 June 2008 * DOI:

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not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * alpha-lipoic acid * diabetic cystopathy * diabetes

mellitus * neuropathy * bladder * oxidative stress * nerve growth factor * rats