Limitations of the nested reverse transcriptase polymerase chain reaction on tyrosinase for the detection of malignant melanoma micrometastases in lymph nodes

The specificity and sensitivity of the nested reverse transcriptase polymerase chain reaction (RT-PCR) on tyrosinase was studied, for the detection of micrometastases of malignant melanoma.

The specificity was assessed in the blood of six healthy donors, four patients with non-melanoma cancers of which one patient was treated with granulocyte-colony stimulating factor. Lymph

nodes of nine patients without malignant melanoma were tested and four cell lines of various other tumours. Six of the nine non-melanoma lymph nodes were positive in this assay. The

sensitivity was tested in a spike experiment in vitro, using a melanoma cell line. The detection limit was ten melanoma cells per 107 peripheral blood lymphocytes. © 2000 Cancer Research

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Andrews PW, Damjanov I, Simon D, Banting GS, Carlin C, Dracopoli NC and Fogh J (1984) Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line tera-2.

Differentiation in vivo and in vitro. J Lab Invest 50: 147–162

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