Transcriptional profiling of the peripheral blood response during tularemia

Transcriptional profiling of the peripheral blood response during tularemia


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ABSTRACT Tularemia is a febrile disease caused by the highly contagious bacterium _Francisella tularensis_. We undertook an analysis of the transcriptional response in peripheral blood


during the course of ulceroglandular tularemia by use of Affymetrix microarrays comprising 14 500 genes. Samples were obtained from seven individuals at five occasions during 2 weeks after


the first hospital visit and convalescent samples 3 months later. In total, 265 genes were differentially expressed, 95 of which at more than one time point. The differential expression was


verified with real-time quantitative polymerase chain reaction for 36 genes (_R_2=0.590). The most prominent changes were noted in samples drawn on days 2–3 and a considerable proportion of


the upregulated genes appeared to represent an interferon-_γ_-induced response and also a proapoptotic response. Genes involved in the generation of innate and acquired immune responses were


found to be downregulated, presumably a pathogen-induced event. A logistic regression analysis revealed that seven genes were good predictors of the early phase of tularemia. This is the


first description of the transcriptional host response to ulceroglandular tularemia and the study has identified gene subsets relevant to the pathogenesis of the disease and subsets that may


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institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS COMPARISON OF HOST IMMUNE RESPONSES TO LPS IN HUMAN USING AN IMMUNE PROFILING


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Västerbottens läns landsting, and the Medical Faculty, Umeå University, Umeå, Sweden, DARPA, Grant LN00A033 from Ministry of Education, Sport and Youth, Czech Republic. AUTHOR INFORMATION


AUTHORS AND AFFILIATIONS * Department of Clinical Microbiology, Clinical Bacteriology, Umeå University, Umeå, Sweden H Andersson, B Hartmanová, M Landfors, L Näslund, P Rydén & A


Sjöstedt * Department of Infectious Diseases, Örebro University Hospital, Örebro, Sweden E Bäck & H Eliasson * Faculty of Military Health Science, Proteome Center for the Study of


Intracellular Parasitism of Bacteria, University of Defence, Hradec Králové, Czech Republic B Hartmanová Authors * H Andersson View author publications You can also search for this author


inPubMed Google Scholar * B Hartmanová View author publications You can also search for this author inPubMed Google Scholar * E Bäck View author publications You can also search for this


author inPubMed Google Scholar * H Eliasson View author publications You can also search for this author inPubMed Google Scholar * M Landfors View author publications You can also search for


this author inPubMed Google Scholar * L Näslund View author publications You can also search for this author inPubMed Google Scholar * P Rydén View author publications You can also search


for this author inPubMed Google Scholar * A Sjöstedt View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to A Sjöstedt.


RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Andersson, H., Hartmanová, B., Bäck, E. _et al._ Transcriptional profiling of the peripheral blood


response during tularemia. _Genes Immun_ 7, 503–513 (2006). https://doi.org/10.1038/sj.gene.6364321 Download citation * Received: 01 February 2006 * Accepted: 30 May 2006 * Published: 06


July 2006 * Issue Date: 01 September 2006 * DOI: https://doi.org/10.1038/sj.gene.6364321 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get


shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * tularemia


* host response * microarray * IFN-_γ_-induced * diagnostic markers