Derivatives of monoglycerides as apoptotic agents in t-cells

ABSTRACT Recently, lipids have received considerable attention for their potential to induce apoptosis when added exogenously to cells. In this study, we directly demonstrate that murine

T-cells undergo rapid apoptosis following treatment with various forms of monoglycerides, which are a family of naturally occurring lipids consisting of a single fatty acid moiety attached

to a glycerol backbone. The potency of these lipids varied depending on their chemical structure, whereas glycerol backbone or corresponding fatty acids alone were ineffective. Moreover,

monoglyceride-mediated apoptosis was suppressed either by Bcl-2 overexpression, treatment with a broad inhibitor of caspases, or RNA and protein synthesis inhibitors. In addition, treatment

of cells with derivatives of monoglycerides induced a calcium flux, which could be inhibited by both extracellular (EGTA) or intracellular (EGTA-AM) calcium chelators. To our knowledge, this

is the first report demonstrating a role for derivatives of monoglycerides as inducers of apoptosis in mammalian cells. _Cell Death and Differentiation_ (2001) 8, 1103–1112 SIMILAR CONTENT

BEING VIEWED BY OTHERS SUPPRESSION OF HUMAN T CELL ACTIVATION BY DERIVATIVES OF GLYCEROL MONOLAURATE Article Open access 26 April 2021 GLYCEROL MONOLAURATE INHIBITION OF HUMAN B CELL

ACTIVATION Article Open access 05 August 2022 THE INTEGRATION OF METABOLIC AND PROTEOMIC DATA UNCOVERS AN AUGMENTATION OF THE SPHINGOLIPID BIOSYNTHESIS PATHWAY DURING T-CELL DIFFERENTIATION

Article Open access 23 May 2024 INTRODUCTION Apoptosis, or programmed cell death, is a naturally occurring process of cell ‘suicide’ essential for the development and maintenance of tissue

homeostasis in multicellular organisms.1,2 For instance, it is responsible for eliminating cells that have been either overproduced, improperly developed or genetically damaged. It is well

recognized that deregulation of the apoptotic pathway can be involved in the pathogenesis of a variety of diseases such as cancer, AIDS, and neurodegenerative disorders.1 Thus, it is

critical to improve our understanding of the mechanisms regulating apoptosis and to identify new pro- and anti-apoptotic stimuli in order to better design specific therapies. A number of

apoptotic stimuli have been identified, such as ligation of surface receptors (e.g. members of the tumor necrosis factor receptor family, and CD45),3,4,5,6 ligation of intracellular

receptors (e.g. glucocorticoid receptors),7 and exposure to many environmental stresses (e.g. oxidative stress, ultraviolet and γ-irradiation).8,9,10,11,12,13 Furthermore, in recent years,

lipid derivatives have received considerable attention for their potential to induce apoptosis when added exogenously to cells. Most notably, treatment of various cell types with ceramides

or sphingosines have been shown to induce apoptosis.14,15 Addition of either GD3 ganglioside,16 or the alkyllysophospholipid edelfosine

(1-_O_-octadecyl-2-_O_-methyl-_rac_-glycero-3-phosphocholine; ET-18-OCH3)17,18 to leukemic cells has also been reported to trigger apoptosis. Moreover, tributyrin, a triglyceride analog of

the short chain fatty acid butyrate, was demonstrated to induce cell death in MCF-7 human mammary carcinoma cells.19 Also, lysophosphatidic acid has been shown to induce cell death in neural

cells,20,21 and to bind to G protein-coupled receptors of the endothelial differentiation gene family.22,23 Noteworthy, these endothelial differentiation gene family receptors display

homology to the cannabinoid receptors,24,25 which bind lipidic cannabinoid ligands such as Δ9-tetrahydrocannabinol, anandamide, and 2-arachidonyl-glycerol.26,27,28,29 Recently, such

cannabinoids were demonstrated to display pro-apoptotic activity and suggested to have anti-tumoral activity.30,31,32,33,34,35,36 Although these examples do not comprise an exhaustive list,

they illustrate the potency of different lipid derivatives to induce apoptosis. The monoglyceride (MG) family of lipids, consisting of a single fatty acid moiety (saturated or unsaturated)

attached to a glycerol backbone, is much less characterized.37 Under normal physiological conditions, the total concentration of MGs ranges from 2 to 10 μM, whilst the combined concentration

of 1-C16:0 and 1-C18:1 MGs, alone, can reach up to 4 μM in specific tissues.37,38 However, there is no information on the local concentrations of MGs in defined microenvironments, and it is

possible that they could be dramatically increased under pathological conditions as shown with other lipids.39,40 In light of the recent literature on the ability of lipid derivatives to

induce cell death, our objective was to investigate the potential of MGs to induce apoptosis. In this study, we provide direct evidence that derivatives of MGs can rapidly induce apoptosis

in mammalian cells (murine T-cells). We also report that MG-induced apoptosis involves the activation of caspases and can be inhibited by overexpression of Bcl-2, or the addition of either

RNA or protein synthesis inhibitors. RESULTS INDUCTION OF RAPID CELL DEATH IN THYMOCYTES BY SELECTED MONOGLYCERIDES To verify whether treatment of mammalian cells with MGs would induce cell

death, we exposed freshly isolated immature T cells (thymocytes) to various MG-derivatives. Given that MGs are a large family, we used several compounds that differ in the length of the

fatty acid and the degree of unsaturation of the fatty acid attached to the glycerol backbone. Among the various saturated MGs tested, 1-C14:0 MG and 1-C16:0 MG molecules appeared to be the

most potent inducers of cell death, while other saturated MGs such as 1-C10:0 MG, 1-C12:0 MG, and 1-C18:0 MG were less active or even inactive (1-C8:0 MG) (Figure 1). We also assessed

whether the presence of an unsaturated fatty acid bound to the glycerol backbone would influence the potency of MGs to trigger cell death, and found that unsaturated MGs (1-C16:1 MG, 1-C18:1

MG, and 1-C18:2 MG) were even better inducers of cell death (Figure 1). Importantly, no cell death was found when thymocytes were incubated with the vehicle, the glycerol backbone, or the

corresponding fatty acid moieties alone (Figure 1). Finally, we also tested diacylglycerols (DAGs), related compounds to MGs, and observed that whereas 1,2-C8:0 DAG was able to induce cell

death, 1,2-C18:1 DAG was not (Figure 1). This is in contrast to the potencies demonstrated with the corresponding MGs, as 1-C8:0 MG was a poor inducer, while 1-C18:1 MG was a strong death

inducer (Figure 1). Although, at first glance, it appears that only long chain fatty acids containing compounds induce cell death, this is not the case as 1-C18:0 MG, 1-C20:0 MG, 1,2-C18:1

DAG, and long chain fatty acids alone are quite inefficient, while 1,2-C8:0 DAG, a short chain fatty acid containing compound, is a potent inducer of cell death (Figure 1 and not shown).

Therefore, these results clearly demonstrate the ability of MGs to induce cell death in thymocytes and suggest a direct relationship between their chemical structure and their potency.

DERIVATIVES OF MGS INDUCE CALCIUM FLUXES Intracellular calcium flux has been reported to play a role in many pathways which lead to cell death.41,42 Also, exposure to many lipidic compounds,

whether or not they induce cell death, have been shown to be capable of mobilizing intracellular calcium.22,30,43,44 We observed a good correlation between the ability of MGs and DAG to

induce cell death and to mobilize calcium (Figure 2). Notably, the results obtained demonstrated differences in the kinetics as well as in the intensity of the calcium flux induced by

various lipids tested (Figure 2 left panels). Cells treated with lipids in the presence of the extracellular calcium chelator, EGTA, displayed a dramatically reduced calcium flux (Figure 2

right panels). This calcium flux induction was further inhibited in the presence of intracellular calcium chelator, EGTA-AM (Figure 2 inset). Therefore, these results suggest that calcium

from extracellular and intracellular source are involved in MG-induced calcium flux. As the potential of MGs to induce a calcium flux correlated well with their potential to induce cell

death (Figures 1 and 2), experiments were performed to elucidate whether calcium was essential for the induction of cell death by MGs. The results demonstrated that inhibiting a MG-induced

increase in calcium with either EGTA, medium without calcium, EGTA-AM, or various combinations of these conditions could not block cell death as measured by a decrease in mitochondrial

transmembrane potential (ΔΨm), which is one hallmark of cells undergoing cell death (not shown). Hence, these results suggest that calcium mobilization might be involved, whilst not

essential for cell death induced by MGs. CHARACTERISTICS OF MG-INDUCED CELL DEATH Studies described hereafter were performed with 1-C18:1 MG, i.e. oleic acid bound to the glycerol backbone,

because it is a predominant MG found in mammalian tissues.37 Our results demonstrated that thymocytes exposed to 1-C18:1 MG underwent very rapid induction of cell death (within 30 min) in a

dose-dependent manner (Figure 3A,B). These results contrast the longer incubation times required for dexamethasone-mediated cell death, which is a well known inducer of apoptosis in

thymocytes.7,45 We later assessed if cell death induced by MGs involved apoptosis. Treatment of thymocytes with 1-C18:1 MG was shown to trigger a decrease in ΔΨm and was associated with the

generation of reactive oxygen species (ROS) (Figure 4A). In addition, stimulation of thymocytes with 1-C18:1 MG induced: externalization of phosphatidylserine (PS) residues; loosening of

membrane phospholipids; cell shrinkage as observed by forward and side scatter profile using a flow cytometer, which detects size and granularity of cells, respectively; and ultimately DNA

degradation as detected either by the TdT-dependent dUTP-biotin nick end-labeling (TUNEL) assay or the direct visualization of the characteristic DNA nucleosomal ladder (Figure 4A,B,C).

Similar observations were made with cells cultured with saturated 3-C16:0 MG and unsaturated 1-C16:1 MG (not shown). These results clearly indicate that exposure of thymocytes to MGs causes

cell death through the induction of apoptosis. Whereas some agents that induce apoptosis require _de novo_ mRNA and protein synthesis (e.g. dexamethasone), others do not (e.g. Fas).2 1-C18:1

MG-mediated cell death resembles the former class of apoptotic agents, as it was significantly decreased in the presence of either actinomycin D or cycloheximide, RNA or protein synthesis

inhibitors, respectively (Figure 5). We next investigated whether MG-induced apoptosis was also dependent on caspases. The decrease in ΔΨm and PS exposure induced by 1-C18:1 MG was inhibited

with the addition of z-VAD-fmk, a broad inhibitor of caspases, thus suggesting that caspases are activated during MG-induced apoptosis (Figure 6A). Indeed, cleavage of the effector caspase,

caspase 3, was found after 1-C18:1 MG treatment, a process that could also be inhibited with the addition of z-VAD-fmk (Figure 6B). Given that z-VAD-fmk was able to inhibit the decrease in

ΔΨm after 1-C18:1 MG treatment, this suggested that initiator caspases were involved in 1-C18:1 MG-induced cell death. Therefore, we investigated a possible role for the initiator caspases 8

and 9. We observed that caspase 8 was not cleaved after 1-C18:1 MG treatment as measured by Western blot, although it was cleaved in our positive control (i.e. murine T-cell hybridomas

induced to undergo T cell activation-induced cell death which involves Fas/Fas ligand interactions and leads to caspase 8 activation)46,47,48 (Figure 6C). However, activation of caspases

with LEHD substrate specificity (which caspase 9 belongs to) was observed after 1-C18:1 MG treatment using two different caspase activity assay kits (Figure 6D,E). Finally, kinetic studies

were conducted for caspases with DEVD- (which caspase 3 belongs to) and LEHD-ase activity and our results demonstrated that thymocytes treated with 1-C18:1 MG activated caspases very rapidly

(within 15 min), with a slightly higher percentage of cells displaying LEHD-ase activity at 15 min than DEVD-ase activity (Figure 6E). These results contrast the longer incubation times

required for dexamethasone-induced caspase activation (Figure 6E), and correlate well with the kinetics of cell death induction (Figure 3). Together, these findings suggest that caspase 3,

most likely caspase 9, and not caspase 8 are activated in 1-C18:1 MG-induced apoptosis. To further understand the mechanism of apoptosis induced by MGs, we assessed the involvement of Bcl-2,

which is a well known inhibitor of apoptosis,49,50 in MG-mediated cell death. Thymocytes from Bcl-2 transgenic mice were less sensitive to MG treatment than were thymocytes from wild-type

mice (Figure 7A). This protective effect was even more pronounced in murine T-cell hybridoma (DO11.10) cells that overexpress Bcl-2 (Figure 7B). Together, the data indicate that MG-induced

cell death involves changes characteristic of apoptosis, through a pathway involving caspases, which can be inhibited by RNA or protein synthesis inhibitors, or by Bcl-2 overexpression.

DISCUSSION In the present study, we identified derivatives of MGs as novel apoptotic agents in mammalian cells. We noted that T cells treated with several types of MGs underwent rapid

apoptosis in a dose-dependent manner. This property of MGs appears to be specific, since treatment with either glycerol or the corresponding fatty acids did not induce cell death.

Importantly, concentrations at which MGs were found to cause apoptosis were in the same range, and frequently lower than those used with other classes of pro-apoptotic lipids. For instance,

ceramide, sphingosine, GD3 ganglioside, palmitic acid, arachidonic acid, and tributyrin have been studied, in terms of their pro-apoptotic activities, mainly with concentrations ranging from

20 to 500 μM,15,16,51,52,53,54,55 and even up to 5 mM.19 Moreover, under normal physiological conditions, the total concentration of MGs are in the lower micromolar range in specific

tissues.37,38 It is conceivable that under certain stress conditions, MG levels could locally increase transiently as shown with other lipids.14,16 However, this remains to be investigated

and is beyond the scope of this study. Calcium has been implicated in the regulation of apoptosis in a variety of studies.41,42 For instance, it has been shown that the disruption of calcium

homeostasis can trigger apoptosis,56 that some apoptotic pathways lead to an increase in the concentration of intracellular calcium,57 and that calcium chelators inhibit cell death in some

systems,58 but not all.34,59,60 We observed that MGs induce a sustained elevation of intracellular calcium which implicates both a flux from extracellular source as well as mobilization of

intracellular stores (Figure 2). Of interest are the distinct kinetics and intensity in intracellular calcium rises seen with the different MGs. A sustained increase in calcium flux has been

observed by other apoptotic stimuli34 and it has been suggested that prolonged increases in calcium are generally toxic.61 Nevertheless, while derivatives of MGs induce a calcium flux, they

do not appear, under the conditions tested, to be essential for cell death induction, as also shown with other apoptotic experimental systems.34,59,60,62 The exact mechanism by which MGs

induce apoptosis is currently unknown. It is conceivable that MGs could simply induce cell death through a non-specific effect. For instance, MGs could intercalate into the cytoplasmic

membrane altering its fluidity and permeability, as suggested for other lipids,53 which could lead to apoptosis. However, we do not favor this possibility because this property increases

with the lipid melting point.53 In contrast, in our study, unsaturated MGs (lower melting point) displayed, in general, greater or equal potency to trigger apoptosis than their saturated

counterparts (higher melting point) (Figure 1). In addition, the potency of MGs to induce apoptosis varied depending on their chemical structure and, in fact, 1-C8:0 MG had no effect while

1-C18:1 MG was highly potent (Figure 1). Nevertheless, all the corresponding fatty acids tested, used at the same concentrations as MGs, were unable to induce cell death (Figure 1). Also,

defined subsets of early T-cell precursors (CD4− CD8− thymocytes) and mature T cells are relatively resistant to the effect of MGs as compared to the immature subset (CD4+ CD8+ thymocytes),

thus showing that susceptibility to the compound is modulated during T cell differentiation (manuscript in preparation). Moreover, MG-induced cell death requires _de novo_ protein synthesis

and can be blocked by either Bcl-2 overexpression or by the addition of z-VAD-fmk. Finally, we believe that MGs do not cause cell death through a ‘detergent effect’ (i.e. disrupting the cell

membrane and causing rapid cytolysis), given that MGs do not lyse sheep red blood cells (unpublished observations), MG-induced cell death requires _de novo_ mRNA and protein synthesis, and

MG-induced cell death displays cellular specificity which is not the case with other amphiphilic detergents.63,64,65,66,67 Alternatively, MGs used in this study could interact with membrane

receptors to induce apoptosis. Supporting this notion, 2-arachidonyl-glycerol, a MG, has been shown to bind to cannabinoid receptors.28,29 Interestingly, it has been recently reported that

cannabinoids have the potential to induce apoptosis and could even have anti-tumoral activity.30,31,32,33,34,35,36 In addition, lysophosphatidic acid, which resembles MGs, and can itself be

converted to MGs,68 acts through cell surface G protein-coupled receptors of the endothelial differentiation gene family.22 However, preliminary results from our laboratory indicate that

pertussis toxin, an inhibitor of G protein-coupled receptors, is unable to inhibit MG-induced cell death (unpublished observations). Finally, the study of lipids and their mechanism of

actions is hindered due to their amphipathic nature which allows them to bind non-specifically69 and, in fact, the mechanism underlying the action of more extensively studied lipids is still

elusive.18,70,71,72 Regardless of the possible upstream mechanisms, we have shown that treatment with MGs rapidly triggers a cascade of events, such as a drop in ΔΨm, ROS production,

membrane alterations, caspase activation, and finally DNA degradation. In fact, activation of caspases parallels well with cell death induction. Whereas caspases are activated very rapidly

(within 15 min), even before cell death, after MG treatment, dexamethasone requires longer incubation times to activate caspases and to induce cell death (Figures 3 and 6). In addition, the

rapid caspase activation induced by MGs culminates in cellular apoptosis, which can be inhibited by Bcl-2 overexpression. Our findings also indicate that inhibitors of RNA and protein

synthesis could reduce cell death induced by 1-C18:1 MG (Figure 5), suggesting that 1-C18:1 MG-induced apoptosis relies on target molecules. This study demonstrates that treatment with

derivatives of MGs can induce apoptosis in mammalian cells, suggesting that MGs could represent putative anti-tumor agents. For instance, ether lipids, which have some structural similarity

to naturally occurring MGs, have been extensively studied for their anti-cancer activity.73 Among these, edelfosine, a synthetic analog of lysophosphatidylcholine which induces

apoptosis,17,18 has become the prototype for ether lipid anti-tumor drugs, although its mechanism of action is still unclear.18,71,72 Furthermore, several years ago, Kato _et al._74 reported

_in vivo_ anti-tumor activity for MGs, where treatment of mice with MGs significantly inhibited the growth of intraperitoneally implanted Ehrlich ascites tumors, without being toxic for the

host. However, the mechanism of MG-mediated tumor growth inhibition was totally unknown and could, theoretically, have been attributed to a direct cytotoxic or cytostatic activity towards

the tumor cells or, yet, to an indirect effect on the host immune system, increasing the animal response against the tumor cells. Results presented in this study support a direct anti-tumor

activity of MGs. Indeed, preliminary results from our laboratory demonstrate that MGs can induce cell death in leukemic cell lines (manuscript in preparation). Altogether, these findings

suggest that MGs could represent candidate therapeutic agents in the treatment of pathologies such as leukemia. MATERIAL AND METHODS MICE C57BL/6 mice were purchased from the Jackson

Laboratory (Bar Harbor, ME, USA) and Bcl-2 transgenic mice were kindly provided by Dr. P Jolicoeur (Institut de Recherches Cliniques de Montréal, QC, Canada). All the mice were bred and

housed either at the Institut de Recherches Cliniques de Montréal specific pathogen free animal facility or at the Biotechnology Research Institute animal facility, according to

institutional guidelines. CELLS AND CONSTRUCTS Immature T cells (i.e. thymocytes) were obtained from 3–8-week-old mice and plated at 4×106 cells/ml. DO11.10 murine T-cell hybridoma cells75

were plated at either 1.6×106 cells/ml or 4×105 cells/ml depending on the experiment. DO11.10 overexpressing the Bcl-2-Enhanced Green Fluorescent Protein (EGFP) (referred as EGFP-Bcl-2

cells) were obtained by electroporation of DO11.10 cells with an EGFP-Bcl-2 fusion construct. This construct was produced by subcloning the human Bcl-2 cDNA (GeneBank # M14745) 3′ of the

EGFP coding region of the pEGFP-C3 plasmid vector (_Bgl_II/_Eco_RI sites) (Clontech, Palo Alto, CA, USA). Stable transfectants were obtained after G418 selection (Mediatech, Herndon, VI,

USA) and EGFP-Bcl-2 expression was confirmed by flow cytometry. All experiments were conducted in RPMI 1640 medium (Wisent Inc., St-Bruno, QC, Canada) supplemented with 5% fetal calf serum

(FCS: Montréal Biotech Inc., Kirkland, QC, Canada), at 37°C and 5% CO2. DRUG TREATMENT The lipid derivatives used were: caprylic acid (C8:0), 1-monocapryloyl-glycerol (1-C8:0), capric acid

(C10:0), 1-monodecanoyl-glycerol (1-C10:0), lauric acid (C12:0), 1-monolauroyl-glycerol (1-c12:0), myristic acid (C14:0), 1-monomyristoyl-glycerol (1-C14:0), palmitic acid (C16:0),

1-monopalmitoyl-glycerol (1-C16:0), 3-monopalmitoyl-glycerol (3-C16:0), stearic acid (C18:0), 1-monostearoyl-glycerol (1-C18:0), palmitoleic acid (C16:1 _[cis]_ 9),

1-monopalmitoleyl-glycerol (1-C16:1 _[cis]_ 9), oleic acid (C18:1), 1-monooleoyl-glycerol (1-C18:1 _[cis]_ 9), linoleic acid (C18:2 _[cis,cis]_ 9,12), 1-monolinoleoyl-glycerol (1-C18:2

_[cis,cis]_ 9,12), and 1,2-dioctanoyl-glycerol (1,2-C8:0), which were purchased from Sigma-Aldrich (Oakville, ON, Canada). Glycerol was from Fisher Scientific Co. (Montreal, QC, Canada) and

1,2-dioleoyl-glycerol (1,2-C18:1) from Biomol Research Laboratories, Inc. (Plymouth Meeting, PA, USA). All lipids were dissolved in 95% ethanol and stock solutions of 10 mM were stored at

−20°C. Before each experiment, stock solutions were left at room temperature for 10 min, pre-warmed at 37°C for 30 min, vortexed, and then dissolved in RPMI 1640-5% FCS pre-warmed at 37°C

for 5 min. The mixture was vigorously vortexed and added at the appropriate concentration. The final concentration of vehicle (ethanol) in cell suspension was 1% v/v or less. Controls with

vehicle alone were included and were without effect on experiments. Noteworthy, to test for potential lack of solubility of the compounds, stock solutions were also subjected to both

filtering through a 0.22 μm membrane and ultracentrifugation at 100 000×_g_ for 1 h in polyallomer tubes. Furthermore, compounds were also dissolved in DMSO and were subjected to the same

type of filtering as above. Equivalent results were obtained for all conditions tested. Dexamethasone (Sigma-Aldrich) was used at a final concentration of 10−6 or 10−7 M for thymocytes or

cell lines, respectively. When T cell receptor (TCR) cross-linking was used to induce apoptosis, 1 μg/ml of anti-TCR β-chain monoclonal antibodies (mAbs), H57-597,76 were first immobilized

on the bottom of 96-well Probind plates (Falcon, BD Biosciences, Oakville, ON, Canada) by a 2 h incubation at room temperature. zVAD-fmk (N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone:

Kamiya Biomedical Co. Seattle, WA, USA) was dissolved in DMSO and added to cells at 50 μM 15 min before the addition of the apoptotic stimulus. Actinomycin D (Sigma-Aldrich) or cycloheximide

(Sigma-Aldrich) were added at 5 or 50 μg/ml, respectively. INTRACELLULAR CALCIUM MEASUREMENT Intracellular calcium flux was measured as described77 with some modifications. Cells, 5×105,

were washed in RPMI 1640 medium supplemented with 0.1% BSA (ICN Biomedicals, Montreal, QC, Canada), and 25 mM HEPES pH 7.4 (Wisent) (RPMI/BSA medium). The cells were resuspended with 100 μl

RPMI/BSA medium supplemented with 3 μM FLUO-3AM (Molecular Probes, Eugene, OR, USA), and incubated in the dark for 45 min at 37°C under 5% CO2. After washings in RPMI containing 5% FCS and

25 mM HEPES pH 7.4 (RPMI 5%), the cells were resuspended in RPMI 5%, or RPMI 5% supplemented with either 10 mM EGTA (extracellular calcium chelator) (Sigma-Aldrich), or 200 μM EGTA-AM

(intracellular calcium chelator) (Calbiochem; San Diego, CA, USA). The samples were warmed to 37°C for 5 min before the beginning of the acquisition, and maintained at 37°C for the duration

of the flow cytometric analysis. Unstimulated cells were analyzed for 1–2 min to establish baseline fluorescence levels, the stimulus was added, and acquisition was resumed for a total of 15

 min. Flow cytometric analysis were performed on a Coulter EPICS XL™ flow cytometer (Beckman Coulter, Ville St-Laurent, QC, Canada) equipped with a 488 nm argon laser and the XL2 software.

FLUO-3 fluorescence was measured at 525 nm and was plotted against time using the WinMDI™ software (Dr. J Trotter, Scripps Research Institute, San Diego, CA, USA). MEASUREMENT OF

MITOCHONDRIAL TRANSMEMBRANE POTENTIAL AND REACTIVE OXYGEN SPECIES Mitochondrial transmembrane potential (ΔΨm) and reactive oxygen species (ROS) production were evaluated by the incorporation

of the dye 3,3′-dihexyloxacarbocyanine iodide (DiOC6(3)) and the conversion of dihydroethidine into ethidium, respectively. Briefly, after treatment, thymocytes were harvested and incubated

in phosphate buffered saline (PBS) containing 40 nM DiOC6(3) and 2 μM dihydroethidine (Molecular Probes) for 15 min at 37°C. Cells were washed and further incubated at 37°C for 30 min in

PBS. After washing, cells were resuspended in PBS and propidium iodide (PI) (Sigma-Aldrich) was added at a final concentration of 10 μg/ml. DiOC6(3), ethidium and PI fluorescence were

detected at 525, 575 and 670 nm, respectively. STAINING OF MEMBRANE PHOSPHOLIPIDS Phosphatidylserine (PS) exposure was detected by the binding of Annexin V (BioDesign International,

Kennebunk, ME, USA), as previously described.78 Briefly, after harvesting, thymocytes were washed and incubated in binding buffer (10 mM HEPES, 150 mM NaCl, 5 mM KCl, 1 mM MgCl2, and 1.8 mM

CaCl2) with fluorescein isothiocyanate (FITC)-conjugated Annexin V, for 5 min at room temperature. Volume was adjusted to 500 μl with binding buffer and PI was added at 10 μg/ml. Loosening

of phospholipid was detected with merocyanin-540 (MC540: Molecular Probes). Cells were resuspended in 100 μl of PBS, 0.1% BSA containing 5 μg/ml MC540. The samples were incubated for 3 min

at room temperature and resuspended in 400 μl of PBS. Flow cytometric analysis was carried out with detectors at 525, 575, and 670 nm for Annexin V-FITFC, MC540 and PI, respectively.

ASSESSMENT OF DNA DEGRADATION The TdT-dependent dUTP-biotin nick end-labeling (TUNEL) assay was used to detect DNA degradation. Cells were harvested and washed with PBS containing 2% FCS and

0.1% sodium azide (PBS-WB). Cells were fixed for 5 min at room temperature in 2% paraformaldehyde, washed in PBS-WB and permeabilized for 2 min at 4°C with 0.1% Triton X-100, 0.1% sodium

citrate, pH 7.4. After three washings in PBS-WB, the samples were resuspended in the TUNEL reaction mixture containing 80 μM ATP (Pharmacia, Uppsala, Sweden), 4 μM biotin-conjugated dUTP

(Boehringer Mannheim, Laval, QC, Canada), and 7.5 U of terminal deoxynucleotidyl transferase (Pharmacia), and incubated for 1 h at 37°C. Incorporation of dUTP-biotin into degraded DNA was

revealed by incubating the cells for 1 h at 4°C with streptavidin-conjugated-phycoerythrin (Life Technologies, Burlington, ON, Canada) in PBS-WB. Samples were analyzed on the flow cytometer

at 575 nm. Furthermore, nucleosomal fragmentation was detected by gel electrophoresis. A solution of 0.5% _N_-lauroylsarcosine (Sigma-Aldrich), 10 mM EDTA, Tris-HCl 50 mM, pH 8.0, was used

to lyse 106 cells. Proteinase K (Sigma-Aldrich) was then added to a final concentration of 0.25 mg/ml, and the samples were incubated overnight at 50°C. After treatment with 20 μg/ml RNase A

(Boehringer Mannheim) for 1 h at 50°C, the samples were electrophoresed through a 1.5% agarose gel and stained with ethidium bromide. WESTERN BLOTTING Treated cells were washed with PBS,

and pellets were resuspended in sample buffer (62.5 mM Tris-HCl, pH 6.8, 10% glycerol, 2% SDS, bromophenol, 10% 2-mercaptoethanol), and then boiled for 5 min. Proteins from 1.25–2.5×106

DO11.10 cells and thymocytes, respectively, were separated on 15% SDS–PAGE and transferred onto PVDF membranes (Immobilon, Millipore, Mississauga, ON, Canada). Blots were blocked for 1 h at

room temperature and incubated overnight at 4°C with either rabbit polyclonal antibody against caspase-379 (gift from Dr. RP Sékaly, Institut de Recherches Cliniques de Montréal, QC,

Canada), or rabbit polyclonal antibody against caspase-8 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA). Detection was achieved with horseradish peroxidase-conjugated anti-rabbit mAb

(Santa Cruz Biotechnology, Inc.), followed by chemiluminescence (SuperSignal® West Pico, Pierce, Rockford, IL, USA). CASPASE ACTIVITY MEASUREMENTS IN EXTRACTS AND INTACT CELLS Caspase

activity using cellular extracts was measured using ApoAlert® Caspase 9/6 fluorescent assay kit (Clontech) according to manufacturer's recommendations. Cell extracts (60 μg of proteins)

were incubated with 250 μM LEHD-aminomethylcoumarin (AMC) peptide substrates. The fluorescence of free AMC, generated as a result of cleavage of the substrate, was monitored over time with

a Cytofluor® fluorometer (PerSeptive Biosystems, Foster City, CA, USA). Fluorometric detection of LEHD-AMC is performed using excitation and emission wavelengths of 380 and 460 nm,

respectively. Caspase activity using intact cells was measured using either Caspa Tag™ Caspase 3 or Caspa Tag™ Caspase 9 activity kits from Intergen Co. (Manhattanville Road, NY, USA).

Treated cells were incubated with fluorochrome-labeled inhibitors of caspases (fam-DEVD-fmk or fam-LEHD-fmk) for 1 h at 37°C under 5% CO2. Cells were washed and resuspended in 400 μl wash

buffer before flow cytometric analysis at 525 nm. ABBREVIATIONS * AMC: aminomethylcoumarin * DAG: diacylglycerol * DiOC6(3): 3,3′-dihexyloxacarbocyanine iodide * EGFP: enhanced green

fluorescent protein * FCS: fetal calf serum * FITC: fluorescein isothiocyanate * mAb: monoclonal antibody * MC540: merocyanin-540 * MG: monoglyceride * ΔΨm: mitochondrial transmembrane

potential * PBS: phosphate buffered saline * PI: propidium iodide * PS: phosphatidylserine * ROS: reactive oxygen species * TCR: T cell receptor * TUNEL: TdT-dependent dUTP-biotin nick

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the thymus _J. Exp. Med._ 186: 1503–1512 Article  CAS  Google Scholar  Download references ACKNOWLEDGEMENTS This work was jointly supported by PROCREA BioSciences Inc. and Medical Research

Council of Canada (MT-4264, Dr. S Pande; MT-12637, Dr. P Hugo). F Philippoussis is a recipient of a studentship from FRSQ/FCAR. We thank M Pagé for excellent technical assistance and Drs. P

Jolicoeur, B Massie, and R-P Sékaly for sharing of their reagents. We are grateful to Drs. LM Obeid, M Prentki, G Sauvageau and D Giannacopoulos for critically reviewing the manuscript.

AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Division of Research & Development, PROCREA BioSciences Inc., 6100 Royalmount, Montréal, H4P 2R2, Québec, Canada F Philippoussis, M Fortin, 

C Arguin, A-M Steff & P Hugo * Institut de Recherches Cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, H2W 1R7, Québec, Canada F Philippoussis, E Przybytkowski, M Fortin, SV

Pande, A-M Steff & P Hugo * Department of Microbiology and Immunology, McGill University, 3775 University Street, Montréal, H3A 2B4, Québec, Canada F Philippoussis & P Hugo Authors *

F Philippoussis View author publications You can also search for this author inPubMed Google Scholar * E Przybytkowski View author publications You can also search for this author inPubMed 

Google Scholar * M Fortin View author publications You can also search for this author inPubMed Google Scholar * C Arguin View author publications You can also search for this author

inPubMed Google Scholar * SV Pande View author publications You can also search for this author inPubMed Google Scholar * A-M Steff View author publications You can also search for this

author inPubMed Google Scholar * P Hugo View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to P Hugo. ADDITIONAL

INFORMATION Edited by G Salvesen RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Philippoussis, F., Przybytkowski, E., Fortin, M. _et al._ Derivatives of

monoglycerides as apoptotic agents in T-cells. _Cell Death Differ_ 8, 1103–1112 (2001). https://doi.org/10.1038/sj.cdd.4400917 Download citation * Received: 22 February 2001 * Revised: 01

June 2001 * Accepted: 12 June 2001 * Published: 30 October 2001 * Issue Date: 01 November 2001 * DOI: https://doi.org/10.1038/sj.cdd.4400917 SHARE THIS ARTICLE Anyone you share the following

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SharedIt content-sharing initiative KEYWORDS * apoptosis * lipids * monoglycerides * thymocytes * cell death