Impairment of muscle function caused by mutations of phosphorylation sites in myosin regulatory light chain

ABSTRACT MYOSIN regulatory light chain is phosphorylated by myosin light chain kinase at conserved serine and threonine residues in a number of species1. Phosphorylation of myosin regulatory

light chain regulates smooth muscle contraction, but appears to have a modulatory role in striated muscle contraction2. We assessed the _in vivo_ role of myosin regulatory light chain

phosphorylation in the striated muscles of _Drosophila melanogaster_ by substituting alanine at each or both conserved myosin light chain kinase-dependent phosphorylation sites, serine 66

and serine 67. We report here that myosin light chain kinase-dependent phosphorylation is not required for myofibrillogenesis or for the development of maximal isometric force in indirect

flight muscles. However, mutants with substitutions at the major phosphorylation site (serine 66) or with the double substitutions had reduced power output in isolated flight muscle fibres

and reduced flight ability, showing that myosin regulatory light chain phosphorylation is a key determinant of the stretch activation response in _Drosophila_. Access through your

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AUTHORS AND AFFILIATIONS * Department of Molecular Genetics, The Ohio State University, Columbus, Ohio, 43210, USA Rutaiwan Tohtong, Melissa Graham & Amanda Simcox * Department of

Molecular Physiology and Biophysics, University of Vermont, Burlington, Vermont, 05405, USA Hiroshi Yamashita, Joe Haeberle & David Maughan Authors * Rutaiwan Tohtong View author

publications You can also search for this author inPubMed Google Scholar * Hiroshi Yamashita View author publications You can also search for this author inPubMed Google Scholar * Melissa

Graham View author publications You can also search for this author inPubMed Google Scholar * Joe Haeberle View author publications You can also search for this author inPubMed Google

Scholar * Amanda Simcox View author publications You can also search for this author inPubMed Google Scholar * David Maughan View author publications You can also search for this author

inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Tohtong, R., Yamashita, H., Graham, M. _et al._ Impairment of muscle function

caused by mutations of phosphorylation sites in myosin regulatory light chain. _Nature_ 374, 650–653 (1995). https://doi.org/10.1038/374650a0 Download citation * Received: 16 January 1995 *

Accepted: 13 February 1995 * Issue Date: 13 April 1995 * DOI: https://doi.org/10.1038/374650a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content:

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