Plasmid-mediated vegf gene transfer induces cardiomyogenesis and reduces myocardial infarct size in sheep

ABSTRACT We have recently reported that in pigs with chronic myocardial ischemia heart transfection with a plasmid encoding the 165 isoform of human vascular endothelial growth factor

(p_VEGF__165_) induces an increase in the mitotic index of adult cardiomyocytes and cardiomyocyte hyperplasia. On these bases we hypothesized that _VEGF_ gene transfer could also modify the

evolution of experimental myocardial infarct. In adult sheep p_VEGF__165_ (3.8 mg, _n_=7) or empty plasmid (_n_=7) was injected intramyocardially 1 h after coronary artery ligation. After 15

days infarct area was 11.3±1.3% of the left ventricle in the VEGF group and 18.2±2.1% in the empty plasmid group (_P_<0.02). The mechanisms involved in infarct size reduction (assessed

in additional sheep at 7 and 10 days after infarction) included an increase in early angiogenesis and arteriogenesis, a decrease in peri-infarct fibrosis, a decrease in myofibroblast

proliferation, enhanced cardiomyoblast proliferation and mitosis of adult cardiomyocytes with occasional cytokinesis. Resting myocardial perfusion (99mTc-sestamibi SPECT) was higher in

VEGF-treated group than in empty plasmid group 15 days after myocardial infarction. We conclude that plasmid-mediated _VEGF_ gene transfer reduces myocardial infarct size by a combination of

effects including neovascular proliferation, modification of fibrosis and cardiomyocyte regeneration. Access through your institution Buy or subscribe This is a preview of subscription

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INDUCES MYOCARDIAL ANGIOGENESIS AND INCREASES LEFT VENTRICULAR EJECTION FRACTION IN ISCHEMIC PORCINE HEART Article Open access 03 December 2024 AAV2-VEGF-B GENE THERAPY FAILED TO INDUCE

ANGIOGENESIS IN ISCHEMIC PORCINE MYOCARDIUM DUE TO INFLAMMATORY RESPONSES Article Open access 07 February 2022 SAFETY AND BIODISTRIBUTION OF XC001 (ENCOBERMINOGENE REZMADENOVEC) GENE THERAPY

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Download references ACKNOWLEDGEMENTS This work was supported by grants from The René G Favaloro University Foundation and by grant PID #268 from the Argentine National Agency for Scientific

and Technological Promotion (ANPCyT), Secretariat for Technology, Science and Productive Innovation (SeTCIP). Drs Crottogini and Laguens are established Investigators of National Council for

Scientific and Technical Investigations (CONICET). We thank technicians Julio Martínez, Fabián Gauna and Marcela Álvarez, veterinarians María Besansón, Pedro Iguain and Marta Tealdo and

animal house personnel Juan Ocampo, Osvaldo Sosa and Juan Mansilla. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Physiology, Favaloro University, Buenos Aires, Argentina G

Vera Janavel & A Crottogini * Department of Pathology, Favaloro University, Buenos Aires, Argentina P Cabeza Meckert, L Cuniberti & R Laguens * Scientific Investigation Commission

(CIC), Buenos Aires Province, Argentina P Cabeza Meckert * Institute of Cardiology and Cardiovascular Surgery of the Favaloro Foundation, Buenos Aires, Argentina A Mele * Bio Sidus, Buenos

Aires, Argentina M Papouchado, N Fernández, A Bercovich, M Criscuolo & C Melo Authors * G Vera Janavel View author publications You can also search for this author inPubMed Google

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Vera Janavel, G., Crottogini, A., Cabeza Meckert, P. _et al._ Plasmid-mediated VEGF gene transfer induces cardiomyogenesis and reduces myocardial infarct size in sheep. _Gene Ther_ 13,

1133–1142 (2006). https://doi.org/10.1038/sj.gt.3302708 Download citation * Received: 20 July 2005 * Revised: 17 November 2005 * Accepted: 18 November 2005 * Published: 06 April 2006 * Issue

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heart regeneration * cardiomyocytes