Production and concentration of pseudotyped hiv-1-based gene transfer vectors

ABSTRACT Strategies to generate highly concentrated HIV-1 vector pseudotypes involving different envelope (Env) proteins including the vesicular stomatitis virus (VSV) G glycoprotein, the

Moloney murine leukemia virus (MLV) 4070A amphotropic Env and the rabies G glycoprotein were established. Virus stocks were prepared by transient transfection using standard cell culture

media or serum-free media. Such stocks were concentrated 50- to 300-fold by ultracentrifugation or by ultrafiltration using Centricon Plus-80 units yielding titers of up to 10_9_transducing

units per milliliter. There was no loss in titer with any of the pseudotypes tested. Thus, like lentiviral vectors pseudotyped with VSV-G, HIV-1-based vectors pseudotyped with the MLV 4070A

amphotropic Env and the rabies G glycoprotein resist inactivation during concentration. This opens up the possibility to generate highly concentrated HIV-1 vector stocks carrying alternative

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FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS THE STABILITY OF ENVELOPE-PSEUDOTYPED LENTIVIRAL VECTORS Article Open access 24 September 2020 ENGINEERED CHO CELLS AS

A NOVEL AAV PRODUCTION PLATFORM FOR GENE THERAPY DELIVERY Article Open access 06 November 2023 AN EFFICIENT PLASMID-BASED SYSTEM FOR THE RECOVERY OF RECOMBINANT VESICULAR STOMATITIS VIRUS

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providing plasmid ALF. I thank Simon Tang and Hideki Mochizuki for their help during the early phase of this work. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Developmental and Metabolic

Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD J Reiser * Louisiana State University Gene Therapy Program, Louisiana

State University School of Medicine, New Orleans, LA, USA J Reiser Authors * J Reiser View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND

PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Reiser, J. Production and concentration of pseudotyped HIV-1-based gene transfer vectors. _Gene Ther_ 7, 910–913

(2000). https://doi.org/10.1038/sj.gt.3301188 Download citation * Received: 02 September 1999 * Accepted: 15 February 2000 * Published: 25 May 2000 * Issue Date: 01 June 2000 * DOI:

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currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * lentiviral vectors * pseudotypes * gene therapy