Inhibition of hiv-1 replication by a tat transdominant negative mutant in human peripheral blood lymphocytes from healthy donors and hiv-1-infected patients

ABSTRACT It was previously shown that a tat mutant (tat22) where cysteine 22 is substituted by glycine behaves as a transdominant negative mutant in Jurkat T cells lytically or latently

infected by HIV-1. In this study we demonstrate that tat22 controls HIV-1 replication in primary cells. This effect was observed both after in vitro infection of peripheral blood mononuclear

cells (PBMCs) from normal donors and after reactivation of the latent infection in PBMCs from seropositive patients. The antiviral effect of tat22 was limited to conditions of low virus

production. The use of tat22 may be promising for a gene therapy approach to AIDS during the asymptomatic phase of the disease allowing control of virus replication in infected cells and

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subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS CLEARANCE OF HIV INFECTION BY SELECTIVE ELIMINATION OF HOST CELLS CAPABLE OF PRODUCING HIV

Article Open access 13 August 2020 TRISPECIFIC ANTIBODY TARGETING HIV-1 AND T CELLS ACTIVATES AND ELIMINATES LATENTLY-INFECTED CELLS IN HIV/SHIV INFECTIONS Article Open access 22 June 2023

THE SARS-COV-2 OMICRON BA.1 SPIKE G446S MUTATION POTENTIATES ANTIVIRAL T-CELL RECOGNITION Article Open access 21 September 2022 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of

Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Via Luigi Borsari 46, Ferrara, I-44100, Italy C Rossi, PG Balboni, M Betti, PC Marconi, R Bozzini, MP

Grossi, G Barbanti-Brodano & A Caputo * Interdepartmental Centre for Biotechnology, University of Ferrara, Via Luigi Borsari 46, Ferrara, I-44100, Italy G Barbanti-Brodano Authors * C

Rossi View author publications You can also search for this author inPubMed Google Scholar * PG Balboni View author publications You can also search for this author inPubMed Google Scholar *

M Betti View author publications You can also search for this author inPubMed Google Scholar * PC Marconi View author publications You can also search for this author inPubMed Google

Scholar * R Bozzini View author publications You can also search for this author inPubMed Google Scholar * MP Grossi View author publications You can also search for this author inPubMed 

Google Scholar * G Barbanti-Brodano View author publications You can also search for this author inPubMed Google Scholar * A Caputo View author publications You can also search for this

author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Rossi, C., Balboni, P., Betti, M. _et al._ Inhibition of HIV-1 replication

by a Tat transdominant negative mutant in human peripheral blood lymphocytes from healthy donors and HIV-1-infected patients. _Gene Ther_ 4, 1261–1269 (1997).

https://doi.org/10.1038/sj.gt.3300522 Download citation * Received: 11 April 1997 * Accepted: 04 July 1997 * Issue Date: 01 November 1997 * DOI: https://doi.org/10.1038/sj.gt.3300522 SHARE

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clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * Tat mutant * peripheral blood lymphocytes * HIV-1 replication