Structural analysis of insertion sequence is5

ABSTRACT Among the transposable DNA elements that have been detected in bacterial genomes and plasmids1–3, the small insertion sequence (IS) elements have been recognized as a class of

autonomous, basic units of transposition. IS elements are not only fully competent of transposition and able to cause several other related chromosomal aberrations such as deletion of

adjacent DNA segments4,5 and integration of circular plasmid DNAs6, but have also proved to be the active units of transposition in the larger composite transposon (Tn) elements1–3,7–9. In

addition, IS and Tn elements interfere with local transcription patterns in the host genome by causing polarity 10,11. Structural and functional analysis of the IS elements should therefore

elucidate both the basis for autonomy of a genetic unit as small as 1,000 base pairs (bp), and the transposition mechanism, including its inherent step of DNA duplication12. We have now

determined the 1,195 bp sequence of the transposable DNA element IS_5_ in phage λKH100. Near-terminal signal structures appear to protect the central region from outside-in transcriptions

and translations, and render IS polarity as a direct consequence of IS autonomy. The nucleotide structure shows an unusual, highly compact organization of two completely overlapping,

antiparallel genes and correlated control sequences. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS

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Open access 21 May 2025 STRUCTURAL INSIGHT INTO TN3 FAMILY TRANSPOSITION MECHANISM Article Open access 18 October 2022 TRANSPOSON-ASSOCIATED TNPB IS A PROGRAMMABLE RNA-GUIDED DNA

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references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institut für Biologie III der Universität Freiburg, Schänzlestrasse 1, D-7800, Freiburg i.Br., FRG Manfred Kröger & Gerd Hobom

Authors * Manfred Kröger View author publications You can also search for this author inPubMed Google Scholar * Gerd Hobom View author publications You can also search for this author

inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Kröger, M., Hobom, G. Structural analysis of insertion sequence IS_5_. _Nature_

297, 159–162 (1982). https://doi.org/10.1038/297159a0 Download citation * Received: 16 March 1981 * Accepted: 18 March 1982 * Issue Date: 13 May 1982 * DOI: https://doi.org/10.1038/297159a0

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