Structural analysis of insertion sequence is5
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ABSTRACT Among the transposable DNA elements that have been detected in bacterial genomes and plasmids1–3, the small insertion sequence (IS) elements have been recognized as a class of
autonomous, basic units of transposition. IS elements are not only fully competent of transposition and able to cause several other related chromosomal aberrations such as deletion of
adjacent DNA segments4,5 and integration of circular plasmid DNAs6, but have also proved to be the active units of transposition in the larger composite transposon (Tn) elements1–3,7–9. In
addition, IS and Tn elements interfere with local transcription patterns in the host genome by causing polarity 10,11. Structural and functional analysis of the IS elements should therefore
elucidate both the basis for autonomy of a genetic unit as small as 1,000 base pairs (bp), and the transposition mechanism, including its inherent step of DNA duplication12. We have now
determined the 1,195 bp sequence of the transposable DNA element IS_5_ in phage λKH100. Near-terminal signal structures appear to protect the central region from outside-in transcriptions
and translations, and render IS polarity as a direct consequence of IS autonomy. The nucleotide structure shows an unusual, highly compact organization of two completely overlapping,
antiparallel genes and correlated control sequences. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS
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institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS THE LATENT CIS-REGULATORY POTENTIAL OF MOBILE DNA IN _ESCHERICHIA COLI_ Article
Open access 21 May 2025 STRUCTURAL INSIGHT INTO TN3 FAMILY TRANSPOSITION MECHANISM Article Open access 18 October 2022 TRANSPOSON-ASSOCIATED TNPB IS A PROGRAMMABLE RNA-GUIDED DNA
ENDONUCLEASE Article Open access 07 October 2021 REFERENCES * Starlinger, P. _Plasmid_ 3, 241–259 (1980). Article CAS Google Scholar * Calos, M. P. & Miller, J. H. _Cell_ 20, 579–595
(1980). Article CAS Google Scholar * Kopecko, D. J. _Progr. molec. subcell. Biol._ 7, 135–234 (1980). Article CAS Google Scholar * Reif, H. J. & Saedler, H. _Molec. gen. Genet._
137, 17–28 (1975). CAS Google Scholar * Kleckner, N., Reichardt, K. & Botstein, D. _J. molec. Biol._ 127, 89–115 (1979). Article CAS Google Scholar * Davidson, N., Deonier, R. C.,
Hu, S. & Ohtsubo, E. in _Microbiology 1974_ (ed. Schlessinger, D.) 56–68 (American Society for Microbiology, Washington DC, 1975). Google Scholar * MacHattie, L. A. & Jackowski, J.
B. in _DNA Insertion Elements, Plasmids, and Episomes_ (eds Bukhari, A. I., Shapiro, J. A. & Adhya, S. L.) 219–228 (Cold Spring Harbor Laboratory, New York, 1977). Google Scholar *
Kleckner, N. & Ross, D. G. _Cold Spring Harb. Symp. quant. Biol._ 43, 1233–1246 (1979). Article CAS Google Scholar * Iida, S., Meyer, J. & Arber, W. _Cold Spring Harb. Symp.
quant. Biol._ 45, 27–43 (1981). Article CAS Google Scholar * Jordan, E., Saedler, H. & Starlinger, P. _Molec. gen. Genet._ 132, 353–363 (1968). Article Google Scholar * Adhya, S. L.
& Gottesmann, M. E. _A. Rev. Biochem._ 47, 967–996 (1978). Article CAS Google Scholar * Lusky, M., Kröger, M. & Hobom, G. _Cold Spring Harb. Symp. quant. Biol._ 45, 173–176
(1981). Article CAS Google Scholar * Blattner, F. R. _et al._ _Virology_ 62, 458–471 (1974). Article CAS Google Scholar * Szybalski, W. in _DNA Insertion Elements, Plasmids, and
Episomes_ (eds Bukhari, A. I., Shapiro, J. A. & Adhya, S. L.) 583–590 (Cold Spring Harbor Laboratory, New York, 1977). Google Scholar * Chow, L. T. & Broker, T. R. _J. Bact._ 133,
1427–1436 (1978). CAS PubMed Google Scholar * Lieb, M. _Gene_ 12, 277–280 (1980). Article CAS Google Scholar * Glansdorff, N. & Sand, G. _Biochim. biophys, Acta_ 108, 308–311
(1965). Article Google Scholar * Crabeel, M., Charlier, D., Cunin, R. & Glansdorff, N. _Gene_ 5, 207–232 (1979). Article CAS Google Scholar * Maxam, A. & Gilbert, W. _Proc.
natn. Acad. Sci. U.S.A._ 74, 560–564 (1977). Article ADS CAS Google Scholar * Sauer, R. T. _Nature_ 276, 301–302 (1978). Article ADS CAS Google Scholar * Landsmann, J., Kröger, M.
& Hobom, G. _Gene_ (in the press). * Kahmann, R. & Kamp, D. _Nature_ 280, 247–250 (1979). Article ADS CAS Google Scholar * Dhar, R., McClements, W. L., Enquist, L. W. & van
de Woude, G. F. _Proc. natn. Acad. Sci. U.S.A._ 77, 3937–3941 (1980). Article ADS CAS Google Scholar * Shoemaker, C. _et al._ _Proc. natn. Acad. Sci. U.S.A._ 77, 3982–3936 (1980).
Article Google Scholar * Shine, J. & Dalgarno, L., _Proc. natn. Acad. Sci. U.S.A._ 71, 1342–1346 (1974). Article ADS CAS Google Scholar * Rosenberg, M. & Court, D. _A. Rev.
Genet._ 13, 319–353 (1979). Article CAS Google Scholar * Rak, B., Lusky, M. & Hable, M. _Nature_ 297, 124–128 (1982). Article ADS CAS Google Scholar * Ghosal, D., Sommer, H. &
Saedler, H. _Nucleic Acids Res._ 6, 1111–1122 (1979). Article CAS Google Scholar * Klaer, R. _et al._ _Cold Spring Harb. Symp. quant. Biol._ 45, 215–224 (1981). Article CAS Google
Scholar * Grindley, N. & Joyce, C. M. _Cold Spring Harb. Symp. quant. Biol._ 45, 125–133 (1981). Article CAS Google Scholar * Ohtsubo, H. & Ohtsubo, E. _Proc. natn. Acad. Sci.
U.S.A._ 75, 615–619 (1978). Article ADS CAS Google Scholar * Johnsrud, L. _Molec. gen. Genet._ 169, 213–218 (1979). Article CAS Google Scholar * Hobom, G. & Lusky, M. in
_Mechanistic Studies of DNA Replication and Genetic Recombination_ (ed. Alberts, B.) 231–255 (Academic, New York, 1980). Book Google Scholar * Langridge, J., Langridge, P. & Bergquist,
P. L. _Analyt. Biochem._ 103, 264–271 (1980). Article CAS Google Scholar * Hobom, G. _et al._ _Cold Spring Harb. Symp. quant. Biol._ 43, 165–178 (1979). Article ADS CAS Google Scholar
* Schoner, B. & Kahn, M. _Gene_ 14, 165–174 (1981). Article CAS Google Scholar * Eugler, J. A. & van Bree, M. P. _Gene_ 14, 155–163 (1981). Article Google Scholar Download
references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institut für Biologie III der Universität Freiburg, Schänzlestrasse 1, D-7800, Freiburg i.Br., FRG Manfred Kröger & Gerd Hobom
Authors * Manfred Kröger View author publications You can also search for this author inPubMed Google Scholar * Gerd Hobom View author publications You can also search for this author
inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Kröger, M., Hobom, G. Structural analysis of insertion sequence IS_5_. _Nature_
297, 159–162 (1982). https://doi.org/10.1038/297159a0 Download citation * Received: 16 March 1981 * Accepted: 18 March 1982 * Issue Date: 13 May 1982 * DOI: https://doi.org/10.1038/297159a0
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