Differential usage of iso-accepting trnaser species in silk glands of bombyx mori

ABSTRACT The rapid development of the silk glands of _Bombyx mori_ during the last larval instar shows two phases. During the first 4 days, in both the middle and posterior parts of the silk

glands, the ribosomal machinery is assembled and the synthesis of house-keeping proteins starts. During the second phase (the last 4 days), the middle part of the gland synthesizes ∼45 mg

of the silk protein sericin (31% serine) and the posterior part of the gland synthesizes ∼130 mg of the silk protein fibroin (46% glycine, 29% alanine and 12% serine). Silk fibroin and

sericin are detectable by the second day and represent 80 and 50% respectively of the total proteins produced at day 8 (refs 1–4). It is known that the tRNA population of the posterior part

of the gland is quantitatively adapted to fibroin codon frequency during this period5–9 but little is known about the situation in the middle part except for the observation that it contains

more tRNASer than does the posterior part. We show here that the two parts contain, and presumably use, different iso-accepting species of tRNASer, the middle part using tRNA1Ser, which

recognizes AGU and AGC codons, and the posterior part using tRNA2Ser which recognizes UCA. We also suggest that this differential adaptation of the tRNASer species is under transcriptional

control as the two species are accumulated at different rates, but degraded at the same rate. Access through your institution Buy or subscribe This is a preview of subscription content,

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ERMINE MOTH, _YPONOMEUTA CAGNAGELLA_, REPRESENTING THE EARLY DIVERGING LINEAGE OF THE DITRYSIAN LEPIDOPTERA Article Open access 23 November 2022 ABSENCE OF FIBROIN H SEQUENCES AND A

SIGNIFICANT DIVERGENCE IN THE PUTATIVE FIBROIN L HOMOLOG IN _NEOMICROPTERYX CORNUTA_ (MICROPTERIGIDAE) SILK Article Open access 13 March 2025 CHARACTERIZATION AND COMPARATIVE ANALYSIS OF

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  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of General and Applied Biology, Université Claude Bernard, Lyon I, 43, Bd du 11 Novembre 1918, F-69622,

Villeurbanne Cedex, France Danièle Hentzen, Anna Chevallier & Jean-Pierre Garel Authors * Danièle Hentzen View author publications You can also search for this author inPubMed Google

Scholar * Anna Chevallier View author publications You can also search for this author inPubMed Google Scholar * Jean-Pierre Garel View author publications You can also search for this

author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Hentzen, D., Chevallier, A. & Garel, JP. Differential usage of

iso-accepting tRNASer species in silk glands of _Bombyx mori_. _Nature_ 290, 267–269 (1981). https://doi.org/10.1038/290267a0 Download citation * Received: 14 July 1980 * Accepted: 09

January 1981 * Issue Date: 19 March 1981 * DOI: https://doi.org/10.1038/290267a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable

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