High resolution detection of dna–rna hybrids in situ by indirect immunofluorescence

ABSTRACT WE describe here a new method for the detection of RNA–DNA hybrids in cytological preparations with which we have revealed the locations of hybrid molecules on polytene chromosomes.

The critical reagent is an antiserum raised in rabbits against poly(rA)·poly(dT) complexed with methylated bovine serum albumin, originally described by Stollar1. The specificity and

resolving power of the indirect immunofluorescence procedure are demonstrated using _in situ_ hybridisation of 5S rRNA (ribosomal RNA) to polytene chromosomes of _Drosophila melanogaster_ as

a model system. The method has significant advantages over the autoradiographic procedures2–5 used so far. Access through your institution Buy or subscribe This is a preview of subscription

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CYTOGENETIC MARKERS ACROSS LEPIDOPTERA Article Open access 09 June 2021 3D MAPPING AND ACCELERATED SUPER-RESOLUTION IMAGING OF THE HUMAN GENOME USING IN SITU SEQUENCING Article 27 July 2020

SIMULTANEOUS VISUALIZATION OF DNA LOCI IN SINGLE CELLS BY COMBINATORIAL MULTI-COLOR IFISH Article Open access 10 February 2022 REFERENCES * Stollar, B. D., _Science_, 169, 609–611 (1970).

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references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania, 19111 GEORGE T. RUDKIN * Department of

Biochemistry, Tufts University School of medicine, Boston, Massachusetts, 02111 B. D. STOLLAR Authors * GEORGE T. RUDKIN View author publications You can also search for this author inPubMed

 Google Scholar * B. D. STOLLAR View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE

THIS ARTICLE RUDKIN, G., STOLLAR, B. High resolution detection of DNA–RNA hybrids _in situ_ by indirect immunofluorescence. _Nature_ 265, 472–473 (1977). https://doi.org/10.1038/265472a0

Download citation * Received: 18 October 1976 * Accepted: 14 December 1976 * Published: 01 February 1977 * Issue Date: 03 February 1977 * DOI: https://doi.org/10.1038/265472a0 SHARE THIS

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