Establishment of a novel human acute myeloblastic leukemia cell line (ynh-1) with t(16;21), t(1;16) and 12q13 translocations

ABSTRACT The t(16;21)(p11;q22) translocation is a non-random chromosomal aberration observed in several types of human acute myeloblastic leukemia (AML), whereas the der(16)t(1;16) and

chromosome rearrangements at 12q13 are frequently found in solid tumors. A novel cell line YNH-1 was established from peripheral blood cells of a 46-year-old male with AML (M1) carrying

t(16;21) and t(1;16) translocations. YNH-1 has been maintained with a doubling time of 82 h for more than 20 months as a granulocyte colony-stimulating factor (G-CSF), granulocyte–macrophage

colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) dependent line. Morphologically YNH-1 cells were free-floating immature myeloblasts with lobulated nuclei and vacuoles in the

cytoplasm. They were positive for myeloperoxidase but negative for α-naphthyl butylate esterase and chloroacetate esterase stainings. In surface marker analysis YNH-1 cells were positive for

CD13, CD33 and CD34. Chromosomal analysis showed 46, XY, der(16)t(16;21)(p11;q22)t(1;16) (q12;q13), der(21)t(16;21)(p11;q22), der(6)t(6;12)(q13;q13), der(12)t(6;12)(q21;q13). These

translocations were confirmed by fluorescence _in situ_ hybridization (FISH) studies with the _ERG_-YAC clone and chromosome-specific DNA libraries. Both the _FUS/ERG_ and _ERG/FUS_ chimeric

transcripts were identified by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Thus, YNH-1 could be a useful tool for elucidating the pathophysiology and molecular

mechanism in AML with t(16;21),t(1;16) and 12q13 translocations. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution

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2023 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Hematology, Musashino Red Cross Hospital, Tokyo, Japan K Yamamoto, H Hamaguchi & K Nagata * SRL, Inc, Tokyo, Japan M

Kobayashi & F Tanimoto * Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan M Taniwaki Authors * K Yamamoto View author publications You can

also search for this author inPubMed Google Scholar * H Hamaguchi View author publications You can also search for this author inPubMed Google Scholar * K Nagata View author publications You

can also search for this author inPubMed Google Scholar * M Kobayashi View author publications You can also search for this author inPubMed Google Scholar * F Tanimoto View author

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PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Yamamoto, K., Hamaguchi, H., Nagata, K. _et al._ Establishment of a novel human acute myeloblastic leukemia cell

line (YNH-1) with t(16;21), t(1;16) and 12q13 translocations. _Leukemia_ 11, 599–608 (1997). https://doi.org/10.1038/sj.leu.2400594 Download citation * Received: 25 September 1996 *

Accepted: 09 December 1996 * Issue Date: 01 April 1997 * DOI: https://doi.org/10.1038/sj.leu.2400594 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

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KEYWORDS * acute myeloblastic leukemia * chromosome translocation * t(16;21) * _FUS/ERG_ transcripts * t(1;16) * 12q13