Regeneration of Bone Marrow Cells and Thymus induced by 19S Alpha-2 Globulin in Irradiated Mice

THE 19S alpha-2 globulin isolated by us from the alpha-2 globulin fraction of human serum (obtained from Mann Res. Lab., New York) by the procedure of Schonenberger et al.1 and identified by

electrophoresis on poly aery lamide gel2 and ultracentrifugation was found to stimulate the regeneration of bone marrow cells in X-irradiated mice (C57Bl/6). A sample (0.5 mg) of the 19S

alpha-2 globulin injected intraperitoneally into 33–35 day old mice within an hour of exposure to 170 r. produced a significant speeding up in the incorporation of 3H-thymidine into bone

marrow cells, as compared with that in irradiated animals injected with saline (Fig. 1). Seven days after irradiation 19S alpha-2 globulin-injected animals showed an actual overshoot in

number of cells of femoral and tibial bone marrow (55 × 106/mouse), as compared with unirradiated controls (42 × 106/mouse) and irradiated but saline-injected controls (41 × 106/mouse),

respectively. The total DNA content of bone marrow cells at this stage showed a similar picture, the femoral and tibial bone marrow cells of mice injected with 19S alpha-2 globulin

containing 527 µg of DNA, while the irradiated (saline-injected) controls contained 410 µg and the non-irradiated controls contained 414 µg of DNA.

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