Thin-layer chromatography of relatively voluminous samples

ABSTRACT THE principle of this method is that a relatively voluminous sample (50–100 µl.) is easily applied over a large area of chromatographic substrate and can then be swept into a narrow

band by an advancing solvent front. This narrow band is used as the starting line for conventional thin-layer chromatography. The width of the band compares favourably with the diameter of

spots applied by the time-consuming, conventional method of repeated application of microlitre amounts. We have used the method for the chromatography of amino-acids in aqueous solvents on

250-µ thick layers of both silica and cellulose, and the method should have general applicability. SIMILAR CONTENT BEING VIEWED BY OTHERS HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY WITH

ELUENTS CONTAINING SDS AIDED BY UV- AND RAMAN SPECTROSCOPY Article Open access 28 April 2025 EXPLICIT RELATION BETWEEN THIN FILM CHROMATOGRAPHY AND COLUMN CHROMATOGRAPHY CONDITIONS FROM

STATISTICS AND MACHINE LEARNING Article Open access 19 January 2025 SILICA NANOLAYER COATED CAPILLARY BY HYDROTHERMAL SOL–GEL PROCESS FOR AMINES SEPARATION AND DETECTION OF TYRAMINE IN FOOD

PRODUCTS Article Open access 06 May 2022 ARTICLE PDF REFERENCES * Bush, I. E., _The Chromatography of Steroids_ (Pergamon Press, Oxford and New York, 1961). Google Scholar  * Yphantis, D.

A., _Ann. N.Y. Acad. Sci._, 88, 586 (1960). Article  ADS  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Division of Biology, California Institute of

Technology, Pasadena, California J. H. FESSLER & HENRIETTE GALLEY Authors * J. H. FESSLER View author publications You can also search for this author inPubMed Google Scholar * HENRIETTE

GALLEY View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE FESSLER, J.,

GALLEY, H. Thin-layer Chromatography of Relatively Voluminous Samples. _Nature_ 201, 1056 (1964). https://doi.org/10.1038/2011056a0 Download citation * Issue Date: 07 March 1964 * DOI:

https://doi.org/10.1038/2011056a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently

available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative