Bcl-xl and rag genes are induced and the response to il-2 enhanced in eμebna-1 transgenic mouse lymphocytes

ABSTRACT We have described transgenic mice expressing Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA-1) in B-cells which show a predisposition to lymphoma. To investigate the underlying

oncogenic mechanisms, we have cross bred transgenic strains of mice, examined the pre-tumour B-cell phenotype and investigated the expression levels of selected cellular genes as a response

to EBNA-1 expression. We have found that _bcl-x__L_ and the recombination activating genes (RAG) 1 and 2 are induced in pre-neoplastic samples of EBNA-1 expressing mice. Induction of

_bcl-x__L_ may explain the observed redundancy in lymphomagenesis between transgenic EBNA-1 and _bcl-2_. In addition, bone marrow cells derived from the EμEBNA-1 mice show a greater capacity

for cultured growth compared to controls, particularly in the presence of IL-2. Notably, _bcl-x__L_ expression is responsive to IL-2. These data shed new light on the potential contribution

of EBNA-1 to EBV associated tumorigenicity as well as to the viral life cycle and open a potential avenue for therapeutic intervention. Access through your institution Buy or subscribe This

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VIRUS-MEDIATED TRANSFORMATION OF B CELLS FROM XIAP-DEFICIENT PATIENTS LEADS TO INCREASED EXPRESSION OF THE TUMOR SUPPRESSOR CADM1 Article Open access 22 October 2022 VIRAL TRANSDUCTION OF

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Download references ACKNOWLEDGEMENTS This work was supported by grants from the Leukaemia Research Fund and the Lady Tata Memorial Trust. The Bcl-2-Ig transgenic mouse line was a kind gift

from S Korsmeyer. Numerous individuals sent plasmid constructs for our use in generating probe fragments for which we are grateful. We thank Bill Cushley for assistance with the FACS

analyses and David Stevenson for critical reading of the manuscript. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Division of Molecular Genetics, Institute of Biomedical and Life Sciences,

University of Glasgow, Glasgow, G11 6NU, UK Penelope Tsimbouri, Mark E Drotar, Joanna L Coy & Joanna B Wilson Authors * Penelope Tsimbouri View author publications You can also search

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to Joanna B Wilson. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Tsimbouri, P., Drotar, M., Coy, J. _et al._ _bcl-x__L_ and RAG genes are induced and

the response to IL-2 enhanced in EμEBNA-1 transgenic mouse lymphocytes. _Oncogene_ 21, 5182–5187 (2002). https://doi.org/10.1038/sj.onc.1205490 Download citation * Received: 17 September

2001 * Revised: 29 March 2002 * Accepted: 31 May 2002 * Published: 29 July 2002 * Issue Date: 01 August 2002 * DOI: https://doi.org/10.1038/sj.onc.1205490 SHARE THIS ARTICLE Anyone you share

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Nature SharedIt content-sharing initiative KEYWORDS * EBV * EBNA-1 * IL-2 * Bcl-xL * transgenic mice * lymphoma