Regulation of cell growth-dependent expression of mammalian cdc6 gene by the cell cycle transcription factor e2f

ABSTRACT CDC6 of _SACCHAROMYCES CEREVISIAE_ regulates the DNA replication initiation through the origin recognition complex (ORC). Identification of a human homolog of the CDC6 gene (HsCdc6)

suggests a universal role of the gene product in DNA replication. Expression of HsCdc6 is growth-regulated. We investigated the molecular basis of growth-regulated expression of mammalian

Cdc6. The promoter activity of isolated HsCdc6 upstream region was activated at late G1 and G1/S boundary in the cell cycle of rat embryonic fibroblast REF52 cells by the addition of serum.

The isolated promoter was activated by exogenous expression of E2F without serum stimulation. However a mutant promoter lacking the E2F recognition sites failed to respond to serum

stimulation and exogenous expression of E2F. Expression of endogenous Cdc6 was induced by exogenous expression of E2F. Therefore, we concluded that the growth-regulated expression of

mammalian Cdc6 was mediated by E2F. Moreover, we demonstrated that exogenous overexpression of either HsCdc6 or HsOrc1 failed to induce DNA synthesis unlike overexpression of E2F1, even

though E2F1 induced both Cdc6 and Orc1, suggesting that E2F may regulate the expression of another gene(s), besides Cdc6 and Orc1, required for induction of cellular DNA synthesis in

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Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS HCF-1 PROMOTES CELL CYCLE PROGRESSION BY REGULATING THE EXPRESSION OF CDC42 Article Open access 23 October 2020 E2F-DEPENDENT

TRANSCRIPTION DETERMINES REPLICATION CAPACITY AND S PHASE LENGTH Article Open access 14 July 2020 CDC7-INDEPENDENT G1/S TRANSITION REVEALED BY TARGETED PROTEIN DEGRADATION Article 04 May

2022 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Human Gene Sciences Center, Graduate School of Dentistry, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan Kiyoshi Ohtani, 

Atsumi Tsujimoto & Masataka Nakamura * Department of Developmental Biology, Graduate School of Dentistry, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan Masa-aki Ikeda

Authors * Kiyoshi Ohtani View author publications You can also search for this author inPubMed Google Scholar * Atsumi Tsujimoto View author publications You can also search for this author

inPubMed Google Scholar * Masa-aki Ikeda View author publications You can also search for this author inPubMed Google Scholar * Masataka Nakamura View author publications You can also search

for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Ohtani, K., Tsujimoto, A., Ikeda, Ma. _et al._ Regulation of

cell growth-dependent expression of mammalian CDC6 gene by the cell cycle transcription factor E2F. _Oncogene_ 17, 1777–1785 (1998). https://doi.org/10.1038/sj.onc.1202105 Download citation

* Received: 02 April 1998 * Revised: 05 May 1998 * Accepted: 05 May 1998 * Published: 09 October 1998 * Issue Date: 08 October 1998 * DOI: https://doi.org/10.1038/sj.onc.1202105 SHARE THIS

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Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * mammalian CDC6 * E2F * promoter * cell cycle * DNA synthesis