Placenta growth factor stimulates map kinase and mitogenicity but not phospholipase c-γ and migration of endothelial cells expressing flt 1

ABSTRACT Vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) are structurally related growth factors for endothelial cells. VEGF binds to the related receptor

tyrosine kinases Flt 1 and KDR/Flk 1 with high affinity, whereas PlGF binds only to Flt 1. Ligand-stimulated KDR is known to transduce signals for cellular activity such as proliferation and

migration, whereas weak or no responses have been recorded for Flt 1. We examined VEGF and PlGF for their capacity to stimulate signal transduction in porcine aortic endothelial cells

expressing Flt 1 or KDR. VEGF had essentially no effect on Flt 1 expressing cells, but induced DNA synthesis and migration of KDR expressing cells. PlGF on the other hand induced DNA

synthesis but not migration of the Flt 1 cells. In agreement, MAP kinase, examined as a marker for DNA synthesis, was activated both by VEGF-stimulation of the KDR cells and by

PlGF-stimulation of the Flt 1 cells. In contrast, phospholipase C-γ (PLC-γ), was tyrosine phosphorylated only in VEGF stimulated KDR cells, and not in the PlGF-stimulated Flt 1 cells, which

is in agreement with a role for PLC-γ in cellular migration. We furthermore examined induction of protein levels of plasminogen activator (PA), which was evident in the PlGF-stimulated Flt 1

cells, but not in the VEGF-stimulated KDR cells. These data show that Flt 1 is able to mediate an array of biological signals when appropriately stimulated and that the pattern of responses

of PlGF-stimulation of Flt 1 is distinct from the pattern of responses to VEGF-stimulation of KDR. Access through your institution Buy or subscribe This is a preview of subscription

content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 50 print issues and online access $259.00 per year only $5.18 per issue

Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL

ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS NRP1 INTERACTS WITH ENDOGLIN AND VEGFR2

TO MODULATE VEGF SIGNALING AND ENDOTHELIAL CELL SPROUTING Article Open access 19 January 2024 HUMAN ENDOTHELIAL CELLS AND FIBROBLASTS EXPRESS AND PRODUCE THE COAGULATION PROTEINS NECESSARY

FOR THROMBIN GENERATION Article Open access 08 November 2021 DIVERSE EFFECTS OF PROSTACYCLIN ON ANGIOGENESIS-RELATED PROCESSES IN THE PORCINE ENDOMETRIUM Article Open access 29 August 2023

AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Dept. of Medical and Physiol. Chemistry Biomedical Center, Box 575, S-751 23, Uppsala, Sweden Eva Landgren, Petter Schiller & Lena

Claesson-Welsh * Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden Yihai Cao Authors * Eva Landgren View author publications You can also search for this

author inPubMed Google Scholar * Petter Schiller View author publications You can also search for this author inPubMed Google Scholar * Yihai Cao View author publications You can also search

for this author inPubMed Google Scholar * Lena Claesson-Welsh View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Landgren, E., Schiller, P., Cao, Y. _et al._ Placenta growth factor stimulates MAP kinase and mitogenicity but not phospholipase C-γ and

migration of endothelial cells expressing Flt 1. _Oncogene_ 16, 359–367 (1998). https://doi.org/10.1038/sj.onc.1201545 Download citation * Received: 12 June 1997 * Revised: 02 September 1997

* Accepted: 03 September 1997 * Published: 26 January 1998 * Issue Date: 22 January 1998 * DOI: https://doi.org/10.1038/sj.onc.1201545 SHARE THIS ARTICLE Anyone you share the following link

with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt

content-sharing initiative KEYWORDS * VEGF * PLGF * endothelial cells * migration * proliferation